PPAR gamma/NR1C3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30473

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PPAR gamma/NR1C3 Primary Antibodies »

Product Specifications

Immunogen

The epitope recognized by NBP3-30473 maps to a region between residue 50 to 100 of human Peroxisome Proliferator-Activated Receptor Gamma using the numbering given in entry NP_056953.2 (GeneID 5468).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPAR gamma/NR1C3 Antibody - BSA Free

PPAR gamma/NR1C3 Antibody

Immunoprecipitation:[NBP3-30473]-

Immunoprecipitation:[NBP3-30473]-Detection of mouse PPAR-gamma by western blot of immunoprecipitates. Samples: Whole cell lysate from differentiated mouse NIH 3T3-L1 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. PPAR-gamma was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated PPAR-gamma, was used at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
PPAR gamma/NR1C3 Antibody - BSA Free

PPAR gamma/NR1C3 Antibody - BSA Free NBP3-30473]-

Whole cell lysate (50 µg) from HeLa, HEK293T,Jurkat, differentiated mouse NIH 3T3-L1, and mouseNIH3T3-L1 cells prepared using NETN lysis buffer.Antibodies: Affinity purified rabbit anti-PPAR-gammaantibody used for WB at0.04 µg/ml. Detection: Chemiluminescence with anexposure time of 75 seconds
PPAR gamma/NR1C3 Antibody - BSA Free

PPAR gamma/NR1C3 Antibody - BSA Free-NBP3-30473-

Whole cell lysate (50 µg) from U937 cellsincubated with (+) or without (-) TPA (200nM, 72 hrs) andlysed using NETN lysis buffer. Antibodies: Affinity purifiedrabbit anti-PPAR-gamma antibody used for WB at 0.1 µg/ml. Detection:Chemiluminescence with an exposure time of 75 seconds.

Applications for PPAR gamma/NR1C3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 µg/mg lysate

Western Blot

1:10,000 - 1:25,000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PPAR gamma/NR1C3

PPAR gamma(PPARG), which encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

Long Name

Peroxisome Proliferator-activated Receptor gamma

Alternate Names

NR1C3, PPARG

Gene Symbol

PPARG

Additional PPAR gamma/NR1C3 Products

Product Documents for PPAR gamma/NR1C3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PPAR gamma/NR1C3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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