PRMT5 Antibody

Novus Biologicals | Catalog # NB100-56641

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PRMT5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a mixture of synthetic peptides corresponding to human PRMT5 amino acid sequence.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRMT5 Antibody

Western Blot: PRMT5 Antibody [NB100-56641]

Western Blot: PRMT5 Antibody [NB100-56641]

Western Blot: PRMT5 Antibody [NB100-56641] - Western blot analysis of PRMT5 in cell lysates from HeLa cells using NB100-56641 at 1:500. Twenty microgram of HeLa cell lysate was loaded per well of a mini gel.
Western Blot: PRMT5 Antibody [NB100-56641]

Western Blot: PRMT5 Antibody [NB100-56641]

Western Blot: PRMT5 Antibody [NB100-56641] - analysis of PRMT5 in A) human HeLa and B) mouse NIH 3T3 lysate using this antibody.

Applications for PRMT5 Antibody

Application
Recommended Usage

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.1 mg/ml BSA.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRMT5

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT5, also known as Janus kinase-binding protein 1 (JBP1) and Skb1Hs (human homolog of Skb1 of fission yeast can catalyze the formation of omega-N(G)-monomethylarginine and symmetric omega-N(G), N(G')-dimethylarginine in a variety of proteins. Recombinant PRMT5 can mono and dimethylate histone 2A and myelin basic protein. PRMT5 appears to have lower specific enzyme activity than PRMT1.

Long Name

Protein arginine N-methyltransferase 5

Alternate Names

EC 2.1.1.320, HRMT1L5, IBP72, JBP1, SKB1, SKB1 homolog

Entrez Gene IDs

10419 (Human)

Gene Symbol

PRMT5

UniProt

O14744 (Human)

Additional PRMT5 Products

Product Documents for PRMT5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PRMT5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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