Prostaglandin J2/PGJ2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-64614

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (50 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL
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Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Specificity

This kit recognizes 15d-PGJ2 in samples. No significant cross-reactivity or interference between 15d-PGJ2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.12

Inter-Assay Precision (Precision between assays) %CV < 5.43

Recovery for Prostaglandin J2/PGJ2 ELISA Kit (Colorimetric)

Recovery

92 - 107%

Linearity

Prostaglandin J2/PGJ2 ELISA Kit (Colorimetric)

Scientific Data Images for Prostaglandin J2/PGJ2 ELISA Kit (Colorimetric)

Prostaglandin J2/PGJ2 ELISA Kit (Colorimetric)

ELISA: Prostaglandin J2/PGJ2 ELISA Kit (Colorimetric) [NBP2-64614] -

ELISA: Prostaglandin J2/PGJ2 ELISA Kit (Colorimetric) [NBP2-64614] - Standard Curve Reference

Kit Contents for Prostaglandin J2/PGJ2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Prostaglandin J2/PGJ2

15-deoxy-D12,14-Prostaglandin J2 (15-d-PGJ2) is one of the ultimate dehydration products of PGD2. PGD2 is formed from PGH2 which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthase. 15-d-PGJ2 induces adipogenesis and is an activator of the gamma isoform of the peroxisome proliferation activated receptor (PPARg).

Additional Prostaglandin J2/PGJ2 Products

Product Documents for Prostaglandin J2/PGJ2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Prostaglandin J2/PGJ2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Prostaglandin J2/PGJ2 ELISA Kit (Colorimetric)

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