Prox1 Antibody (5G10) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80920
![Immunocytochemistry/ Immunofluorescence: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]](https://resources.rndsystems.com/images/products/Prox1-Antibody-5G10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80920-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human, Mouse, Rat, Chicken
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5G10
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
The highly conserved Homeo and Prospero domain of human Prox1. [UniProt# Q92786]
Epitope
The homeo prospero domain of Prox1
Reactivity Notes
It is expected to work with all vertebrates including mammals, birds and fish. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Prox1 Antibody (5G10) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]
Immunocytochemistry/Immunofluorescence: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PROX1 (5G10) NBP1-30045 at a 1:200 dilution overnight at 4C and detected with and anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.![Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]](https://resources.rndsystems.com/images/products/Prox1-Antibody-5G10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80920-img0004.jpg "Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]")
Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]
Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot UD1009o of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic stain![Flow Cytometry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]](https://resources.rndsystems.com/images/products/Prox1-Antibody-5G10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80920-img0005.jpg "Flow Cytometry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]")
Flow Cytometry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]
Flow Cytometry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - HepG2 cells were stained with Prox1 (5G10) NBP1-30045 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody. Image from the standard format of this antibody.![Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]](https://resources.rndsystems.com/images/products/Prox1-Antibody-5G10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80920-img0002.jpg "Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]")
Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]
Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - Rat dentate gyrus showing specific immunolabeling of the prox1 protein. Photo courtesy of Justin Kievits and Teresa Milner, Weill Cornell Medical College. Image from the standard format of this antibody.![Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]](https://resources.rndsystems.com/images/products/Prox1-Antibody-5G10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80920-img0003.jpg "Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]")
Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920]
Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot A of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic staining inApplications for Prox1 Antibody (5G10) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Immunohistochemistry
1:10-1:500
Immunohistochemistry Whole-Mount
reported in scientific literature (PMID 24654984)
Immunohistochemistry-Paraffin
1:100-1:1000
Application Notes
This antibody is CyTOF ready.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Prox1
Long Name
Prospero-related Homeobox 1
Alternate Names
Homeobox prospero-like protein PROX1, prospero homeobox 1, prospero homeobox protein 1, prospero-related homeobox 1, PROX-1
Gene Symbol
PROX1
Additional Prox1 Products
Product Documents for Prox1 Antibody (5G10) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Prox1 Antibody (5G10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Prox1 Antibody (5G10) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review Prox1 Antibody (5G10) - Azide and BSA Free and earn rewards!
Have you used Prox1 Antibody (5G10) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
