PSAP Antibody (MSVA-452M)

Novus Biologicals | Catalog # NBP3-57217

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MSVA-452M
View all PSAP Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human ACPP protein Swissprot P15309

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Prostate: A strong PSAP immunostaining should, be seen in all glandular cells..

Negative Control
Negitve control:Appendix: All epithelial and non-epithelial structures must not show any PSAP immunostaining.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PSAP Antibody (MSVA-452M)

PSAP Antibody (MSVA-452M)

Immunohistochemistry-Paraffin:PSAP Antibody (MSVA-452M)NBP3-57217

Prostate Zusatztext2
PSAP Antibody (MSVA-452M)

Immunohistochemistry-Paraffin:PSAP Antibody (MSVA-452M)NBP3-57217

Prostate Prostatic adenocarcinoma Gleason 448 with strong diffuse PSAP positivity.
PSAP Antibody (MSVA-452M)

Immunohistochemistry-Paraffin:PSAP Antibody (MSVA-452M)NBP3-57217

Appendix mucosa zusatztext 1

Applications for PSAP Antibody (MSVA-452M)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PSAP

PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D, which are similar to each other and are sphingolipid hydrolase activator proteins. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in PSAP gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy.

Long Name

Prosaposin

Alternate Names

GLBA, SAP1

Gene Symbol

PSAP

Additional PSAP Products

Product Documents for PSAP Antibody (MSVA-452M)

Certificate of Analysis

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Product Specific Notices for PSAP Antibody (MSVA-452M)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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