PSMC3 Antibody (1B9) - Azide and BSA Free

Novus Biologicals | Catalog # H00005702-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B9

Format

Azide and BSA Free
View all PSMC3 Primary Antibodies »

Product Specifications

Immunogen

PSMC3 (AAH08713, 53 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNLLPNIESPVTRQEKMATVWDEAEQDGIGEEVLKMSTEEIIQRTRLLDSEIKIMKSEVLRVTHELQAMKDKIKENSEKIKVNKTLPYLVSNVIELLDVD

Localization

Cytoplasmic and Nuclear

Specificity

PSMC3 - proteasome (prosome, macropain) 26S subunit, ATPase, 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PSMC3 Antibody (1B9) - Azide and BSA Free

Western Blot: PSMC3 Antibody (1B9) [H00005702-M01]

Western Blot: PSMC3 Antibody (1B9) [H00005702-M01]

Western Blot: PSMC3 Antibody (1B9) [H00005702-M01] - PSMC3 monoclonal antibody (M01), clone 1B9 Analysis of PSMC3 expression in HeLa.
Sandwich ELISA: PSMC3 Antibody (1B9) [H00005702-M01] - Detection limit for recombinant GST tagged PSMC3 is 0.03 ng/ml as a capture antibody.

Applications for PSMC3 Antibody (1B9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PSMC3

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.

Alternate Names

human immunodeficiency virus tat transactivator binding protein-1, MGC8487, proteasome (prosome, macropain) 26S subunit, ATPase, 3,26S proteasome AAA-ATPase subunit RPT5, Proteasome 26S subunit ATPase 3, Proteasome subunit P50, TBP-126S protease regulatory subunit 6A, TBP1Tat-binding protein 1

Entrez Gene IDs

5702 (Human)

Gene Symbol

PSMC3

OMIM

186852 (Human)

UniProt

AAH08713 (Human)

Additional PSMC3 Products

Product Documents for PSMC3 Antibody (1B9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PSMC3 Antibody (1B9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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