Rab5a Antibody - BSA Free

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• Rab5a Antibody

  Name: NIKHIL VAD

  Application: Immunofluorescence (IF)

  Sample Tested: A172 Glioblastoma cells

  Species: Human

  Verified Customer | Posted 07/17/2018

  Untreated GBM cells stained with endosome marker Rab5 (green punctate).

  GBM cells grown on uncoated coverslips were fixed with 4% paraformaldehyde (ice cold) for 15 min. Following fixation, cells were rinsed thrice with PBS, permeabilized with ice-cold methanol for 10 min at 4C. Following permeabilization, coverslips were rinsed thrice with PBS, incubated with blocking buffer (5% goat serum, 0.3% Triton-X 100) for 2 h at RT. Samples were then incubated with Rab5 antibody (1:200) at 4C overnight. Following morning, coverslips were washed thrice with PBS, incubated with anti-rabbit Alexa-Fluor 488 secondary antibody (1:200) for 1 h at RT. Following three washes with PBS, coverslips were mounted on slides using Prolong Gold (with DAPI).

  

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