Rad21 Antibody

Novus Biologicals | Catalog # NBP2-20053

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Chicken (94%), Chimpanzee (100%), Rhesus Macaque (100%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Rad21 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rad21. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (90%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad21 Antibody

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Rat kidney. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - C2C12 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - HBL435 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Paraffin-embedded human lung papillary adenocarcinoma. RAD21 antibody diluted at 1:250.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Mouse testis. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: Rad21 Antibody [NBP2-20053]

Immunoprecipitation: Rad21 Antibody [NBP2-20053]

Immunoprecipitation: Rad21 Antibody [NBP2-20053] - Jurkat whole cell extracts using 5 ug of RAD21 antibody. Western blot analysis was performed using RAD21 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Rad21 Antibody

Western Blot: Rad21 Antibody [NBP2-20053] -

Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (NBP2-20053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Rad21 Antibody

Immunocytochemistry/ Immunofluorescence: Rad21 Antibody [NBP2-20053] -

Rad21 antibody detects Rad21 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad21 stained by Rad21 antibody (NBP2-20053) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Rad21 Antibody

Western Blot: Rad21 Antibody [NBP2-20053] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (NBP2-20053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Rad21 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP2-20053 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rad21

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq]

Alternate Names

double-strand-break repair protein rad21 homolog, hHR21FLJ25655, HR21, KIAA0078RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, NXP-1, NXP1HRAD21, protein involved in DNA double-strand break repair, RAD21 homolog (S. pombe), SCC1 homolog, SCC1FLJ40596

Gene Symbol

RAD21

UniProt

O60216 (Human)

Additional Rad21 Products

Product Documents for Rad21 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rad21 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Rad21 Antibody (1)

5 out of 5
1 Customer Rating
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  • Rad21 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: whole cell lysate, Sample Amount: 40 ug
    Species: Human
    Verified Customer | Posted 04/11/2019
    Western blot analysis of meningioma primary cell lysates yielded clear bands at the predicted molecular weight.

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Protocols

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FAQs

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