Rad51 Antibody (5S9B0)

Novus Biologicals | Catalog # NBP3-16149

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5S9B0 expressed in HEK293
View all Rad51 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rad51 (Q06609). MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEII

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad51 Antibody (5S9B0)

Western Blot: Rad51 Antibody (5S9B0) [NBP3-16149]

Western Blot: Rad51 Antibody (5S9B0) [NBP3-16149]

Western Blot: Rad51 Antibody (5S9B0) [NBP3-16149] - Western blot analysis of extracts of various cell lines, using Rad51 Rabbit mAb (NBP3-16149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Rad51 Antibody (5S9B0)

Chromatin Immunoprecipitation: Rad51 Antibody (5S9B0) [NBP3-16149] -

Chromatin Immunoprecipitation: Rad51 Antibody (5S9B0) [NBP3-16149] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using Rad51 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Applications for Rad51 Antibody (5S9B0)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:50-1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rad51

Rad51 is involved in both the recombination and repair of DNA damage. Its function may be modulated by interaction with other repair proteins including BRCA1 and BRCA2. Alterations in the Rad51 gene have been associated with increased cancer risk. Rad51 is conserved across species and is homologous to prokaryotic RecA.

Alternate Names

BRCC5, hRAD51, HsRad51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A

Gene Symbol

RAD51

Additional Rad51 Products

Product Documents for Rad51 Antibody (5S9B0)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rad51 Antibody (5S9B0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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