RAR alpha/NR1B1 Antibody

Western Blot: RAR alpha/NR1B1 Antibody [NBP3-11869]

Western Blot: RAR alpha/NR1B1 Antibody [NBP3-11869] - Human embryonic kidney cells (293T) were transfected with a RAR alpha construct (lane 2) or with a negative control construct (lane 1) and analysed by Western blot using NBP3-11869, diluted 1:750 in BSA/PBS- Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

Chromatin Immunoprecipitation: RAR alpha/NR1B1 Antibody [NBP3-11869] - ChIP assays were performed using NB4 cells, NBP3-11869 and optimized primer pairs for qPCR. Sheared chromatin from 6 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. This image shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.

ELISA: RAR alpha/NR1B1 Antibody [NBP3-11869]

ELISA: RAR alpha/NR1B1 Antibody [NBP3-11869] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of NBP3-11869. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,400.