CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X3-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Rat CX3CL1 cDNA encodes a 393 amino acid (aa) residue precursor protein with two alternative (21 aa or 24 aa residue) putative signal peptides, a 76 aa residue globular chemokine domain, a 238 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 36 aa residue cytoplasmic domain. The extracellular domain of CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, rat CX3CL1 shares a high degree of amino sequence homology (83% sequence identity) with human and mouse CX3CL1. CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up‑regulated on activated endothelial cells. Membrane‑bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T‑lymphocytes but not monocytes. CX3CR1, previously named V28 or chemokine beta receptor‑like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind fractalkine with high-affinity.
Rat CX3CL1/Fractalkine Chemokine Domain Antibody [Biotin]
R&D Systems | Catalog # BAF537
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Key Product Details
Species Reactivity
Rat
Applications
Western Blot, ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant rat CX3CL1/Fractalkine Chemokine Domain
Gln25-Gly100
Accession # O55145
Gln25-Gly100
Accession # O55145
Specificity
Detects rat CX3CL1/Fractalkine Chemokine Domain in ELISAs and Western blots. In sandwich ELISAs, approximately 30% cross-reactivity with recombinant human (rh) Fractalkine (362-CX) is observed and approximately 5% cross-reactivity with recombinant mouse (rm) Fractalkine (571-MF) and rmFractalkine (472-FF) and less than 2% cross-reactivity with rmFractalkine (458-MF) and rhFractalkine (365-FR) is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat CX3CL1/Fractalkine Chemokine Domain Antibody [Biotin]
Rat CX3CL1 / Fractalkine ELISA Standard Curve
Recombinant Rat CX3CL1/Fractalkine (Catalog # 536-FR) was serially diluted and captured by Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Rat CX3CL1/Fractalkine Chemokine Domain Antibody [Biotin]
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Rat CX3CL1/Fractalkine (Catalog # 537-FT)
Sample: Recombinant Rat CX3CL1/Fractalkine (Catalog # 537-FT)
Rat CX3CL1/Fractalkine Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CX3CL1/Fractalkine
References
- Kledal, T.N. et al. (1998) FEBS Lett. 441:209.
- Combadiere, C. et al. (1998) J. Biol. Chem. 273:23799.
- Harrison, J.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10896.
- Rossi, D.L. et al. (1998) Genomics 47:163.
Alternate Names
FKN, Fractalkine, Neurotactin
Gene Symbol
CX3CL1
UniProt
Additional CX3CL1/Fractalkine Products
Product Documents for Rat CX3CL1/Fractalkine Chemokine Domain Antibody [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Rat CX3CL1/Fractalkine Chemokine Domain Antibody [Biotin]
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
