Rat Fetuin A/AHSG Antibody (774761)

R&D Systems | Catalog # MAB7510

R&D Systems

Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 774761
View all Fetuin A/AHSG Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Fetuin A/AHSG
Ala19-I352
Accession # P24090

Specificity

Detects rat Fetuin A/AHSG in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Fetuin A or recombinant mouse Fetuin A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rat Fetuin A/AHSG Antibody (774761)

Detection of Rat Fetuin A/AHSG antibody by Western Blot.

Detection of Rat Fetuin A/AHSG by Western Blot.

Western blot shows lysates of rat liver tissue and rat lung tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat Fetuin A/AHSG Monoclonal Antibody (Catalog # MAB7510) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fetuin A/AHSG at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Rat Fetuin A/AHSG Antibody (774761)

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: Rat liver tissue and rat lung tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fetuin A/AHSG

Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It has been also called "countertrypsin" because of its ability to inhibit trypsin (1). Fetuin A is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (2, 3). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (4). The major form of plasma Fetuin A corresponds to two disulfide bond-linked chains derived from the single chain (5). Fetuin A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300-600 μg/mL) which fall significantly (30-50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (7, 8). Mature rat Fetuin A shares 60% and 85% aa sequence identity with human and mouse Fetuin A, respectively.

Long Name

alpha-2-HS Glycoprotein

Alternate Names

AHSG, alpha-2-HS-glycoprotein

Entrez Gene IDs

197 (Human); 11625 (Mouse); 25373 (Rat)

Gene Symbol

AHSG

UniProt

P24090

Additional Fetuin A/AHSG Products

Product Documents for Rat Fetuin A/AHSG Antibody (774761)

Certificate of Analysis

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Product Specific Notices for Rat Fetuin A/AHSG Antibody (774761)

For research use only

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