Rat IFN-gamma Antibody [Biotin]

R&D Systems | Catalog # BAF585

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IFN-gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat IFN-gamma (R&D Systems, Catalog # 585-IF)
Gln23-Cys156
Accession # P01581

Specificity

Detects rat IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant mouse IFN‑ gamma is observed and less than 0.1% cross-reactivity with recombinant human IFN-gamma and recombinant porcine IFN-gamma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat IFN-gamma Antibody [Biotin]

Rat IFN-gamma ELISA Standard Curve

Rat IFN-gamma ELISA Standard Curve

Recombinant Rat IFN‑ gamma (Catalog # 585-IF) was serially diluted and captured by Mouse Anti-Rat IFN‑ gamma Monoclonal Antibody (Catalog # MAB5851) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Rat IFN-gamma Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rat IFN-gamma (Catalog # 585-IF)

Rat IFN-gamma Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Rat IFN‑ gamma Antibody (Catalog # MAB5851)
  • Standard: Recombinant Rat IFN-gamma Protein (Catalog # 585-IF)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF585 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rat IFN-gamma exists as a noncovalently linked homodimer of 20 - 25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with mouse IFN-gamma and 37% - 45% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN‑ gamma  RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References

  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Dijkema, R. et al. (1985) EMBO J. 4:761.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol

IFNG

UniProt

P01581

Additional IFN-gamma Products

Product Documents for Rat IFN-gamma Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat IFN-gamma Antibody [Biotin]

For research use only

Citations for Rat IFN-gamma Antibody [Biotin]

Customer Reviews for Rat IFN-gamma Antibody [Biotin] (1)

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  • Rat IFN-gamma Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Rat
    Verified Customer | Posted 11/08/2017
    This antibody was used as the detection in an ELISA reaction with MAB585 as the capture to measure rat IFNy in serum samples.
    Rat IFN-gamma Antibody [Biotin] BAF585

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