Rat IL-17/IL-17A Antibody (905515) [Alexa Fluor™ Plus 488]

R&D Systems | Catalog # FAB84101AFP488

R&D Systems

Key Product Details

Species Reactivity

Rat

Applications

ELISA

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 905515
View all IL-17/IL-17A Primary Antibodies »

Product Specifications

Specificity

Detects Rat IL‑17/IL‑17A in direct and capture ELISAs. In direct Elisa, no cross-reactivity with Rat IL17F was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Rat IL-17/IL-17A Antibody (905515) [Alexa Fluor™ Plus 488]

Application
Recommended Usage

ELISA

Optimal dilution of this antibody should be experimentally determined.

Background: IL-17/IL-17A

Rat IL-17A/F is an approximately 37 kDa, secreted, disulfide-linked glycosylated heterodimeric protein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors such as TGF-beta (1). Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 3-5). Mature rat IL-17A and IL-17F share 60% and 59% amino acid sequence identity with human and 61% and 90% with mouse IL-17A and IL-17F, respectively. They also share 50% aa sequence identity with each other (1). IL-17A/F heterodimer and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10, 14). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of
IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).

References

  1. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  2. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  4. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  5. Gu, C. et al. (2013) Cytokine 64:477.
  6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
  7. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  8. Ouyang, W. et al. (2008) Immunity 28:454.
  9. Steinman, L. (2007) Nat. Med. 13:139.
  10. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
  11. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  12. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
  13. Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.
  14. Spolski, R. et al. (2009) Eur. J. Immunol. 3: 658.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Gene Symbol

IL17A

UniProt

Q61453 (Rat)

Additional IL-17/IL-17A Products

Product Documents for Rat IL-17/IL-17A Antibody (905515) [Alexa Fluor™ Plus 488]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat IL-17/IL-17A Antibody (905515) [Alexa Fluor™ Plus 488]


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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