Interleukin-2 (IL-2) is a O-glycosylated four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic cells, and eosinophils (1 - 3). Mature rat IL-2 shares 66% and 73% amino acid sequence identity with human and mouse IL-2, respectively. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (4 - 6). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (7). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (8 - 10). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (11, 12).
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
ELISA Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant rat IL-2 (R&D Systems, Catalog # 502-RL)
Ala21-Gln155
Accession # P17108
Ala21-Gln155
Accession # P17108
Specificity
Detects rat IL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human IL-2 and recombinant mouse IL-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat IL-2 Antibody [Biotin]
Rat IL-2 ELISA Standard Curve
Recombinant Rat IL‑2 (Catalog # 502-RL) was serially diluted and captured by Goat Anti-Human/Rat IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-502-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Rat IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-502-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Rat IL-2 Antibody [Biotin]
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Rat IL‑2 (Catalog # 502-RL)
Sample: Recombinant Rat IL‑2 (Catalog # 502-RL)
Rat IL-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using BAF502 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-2
References
- Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
- Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
- McKnight, A. et al. (1989) Immunogenetics 30:145.
- Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
- Wang, X. et al. (2005) Science 310:1159.
- Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
- Jaleco, S. et al. (2003) J. Immunol. 171:61.
- Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
- Laurence, A. et al. (2007) Immunity 26:371.
- Kryczek, I. et al. (2007) J. Immunol. 178:6730.
- Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
- Fehervari, Z. et al. (2006) Trends Immunol. 27:109.
Long Name
Interleukin 2
Alternate Names
Aldesleukin, IL2, Proleukin, TCGF
Entrez Gene IDs
Gene Symbol
IL2
UniProt
Additional IL-2 Products
Product Documents for Rat IL-2 Antibody [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Rat IL-2 Antibody [Biotin]
For research use only
Related Research Areas
Citations for Rat IL-2 Antibody [Biotin]
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Customer Reviews for Rat IL-2 Antibody [Biotin] (2)
4.5 out of 5
2 Customer Ratings
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Application: ELISASample Tested: SerumSpecies: RatVerified Customer | Posted 11/08/2017This antibody was used as a detection with AF502 as the capture in an ELISA to measure Rat IL-2 in serum samples.
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Application: ELISASample Tested: Cell culture supernatantVerified Customer | Posted 10/26/2015ELISA plates were coated by incubating the diluted capture antibody (anti-rat IL2,R&D systems) overnight at 4C. To detect the plate-bound IL2 from the culture supernatant, the detection antibody (biotin anti-rat IL2) was used at 400ng/mL, followed by avidin-HRP. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: blocking buffer, wash buffer<br />Dilution: 400ng/mL
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Innate Lymphoid Cell Differentiation Pathways
Jak/STAT Signaling Pathway
Th1 Differentiation Pathway
Th2 Differentiation Pathway
