Rat IL-4 Antibody

Name: Rat IL-4 Antibody
Brand: R&D Systems
SKU: AF-504-NA
Price: 519 USD
Availability: InStock

R&D Systems | Catalog # AF-504-NA

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Citations (5)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IL-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat IL‑4
Cys25-Ser147
Accession # P20096

Specificity

Detects rat IL-4 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant cotton rat IL‑4 and recombinant mouse IL-4 is observed, and less than 1% cross-reactivity with recombinant human IL‑4, recombinant canine IL-4, and recombinant porcine IL‑4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat IL-4 Antibody

Cell Proliferation Induced by IL‑4 and Neutralization by Rat IL‑4 Antibody.

Recombinant Rat IL-4 (Catalog # 504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-4 (4 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA). The ND₅₀ is typically 0.05-015 µg/mL in the presence of PHA (10 µg/mL).

Detection of IL-4 by Western Blot

HFSCs and HFSCs/HGF suppressed inflammatory reaction. A–E Iba-1 staining in the penumbra at 7 days after cell transplantation. Scale bar = 100 µm. F The quantitative analysis of the numbers of Iba-1-positive cells. G Representative western blot bands of inflammation-related proteins in different groups. H and I Quantification of TNF-a and IL-4 expression in the ischemic hemisphere extracts. Values are the mean ± SD. *P < 0.05 Saline group vs. Control group, #P < 0.05 HFSCs group vs. Saline group, ∆P < 0.05 HFSCs/HGF group vs. HFSCs/Vehicle group, n = 6 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36782269), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IL-4 by Western Blot

Rat IL-4 ELISA Standard Curve

Recombinant Rat IL‑4 (Catalog # 504-RL) was serially diluted and captured by Mouse Anti-Rat IL‑4 Monoclonal Antibody (Catalog # MAB504) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

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Applications for Rat IL-4 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rat IL‑4 (Catalog # 504-RL)

Neutralization

Measured by its ability to neutralize IL‑4-induced proliferation in the rat splenocytes. The Neutralization Dose (ND₅₀) is typically 0.05-015 µg/mL in the presence of 4 ng/mL Recombinant Rat IL‑4 and 10 µg/mL PHA.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rat IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature rat IL-4 shares 41%, 43%, and 59% aa sequence identity with bovine, human, and mouse IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in rodent B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).