Rat ROBO1 Antibody

Detection of ROBO1 by Immunohistochemistry

Elevated expression of Robo1 leads to a decrease in IPC proliferation.(A) In utero electroporation (IUE) of either an empty vector (control) or a Robo1 expression vector (Robo1 OE) together with a H3-GFP expression vector was performed into WT cortices at E13.5. The cortical sections are stained with DAPI (blue), anti-GFP (green), anti-PH3 (magenta), and anti-TBR2 (white) antibodies at E15.5. The graph shows the ratio of PH3+ cells in TBR2+ and GFP+ cells (left) or PH3+ cells in TBR2+ and GFP-negative cells (right). Arrows indicate GFP+, PH3+, and TBR2+ cells. (B) IUE of an empty (control) or a Robo1 expression vector (Robo1 OE) and a H3-GFP expression vector into WT cortices at E14.5. The cortical sections are immunostained with DAPI (blue), anti-GFP (green), anti-KI67 (magenta), and anti-TBR2 (white) antibodies at E16.5. The graph shows the percentage of KI67+ and TBR2-negative cells located in the VZ (RGCs), TBR2+ and KI67+ cells (IPCs), and KI67-negative differentiated cells (postmitotic neurons) among total GFP+ cells. The percentage of RGCs and IPCs in electroporated cells was not different between Robo1 OE and control. (C) Auts2fl(ex8)/fl(ex8) or Emx1Cre/+; Auts2fl(ex8)/fl(ex8) (Auts2 cKO) cortices were electroporated with scrambled or Robo1 shRNA vector in utero at E13.5 and immunostained with anti-GFP (green), anti-PH3 (magenta) and anti-TBR2 (white) antibodies at E15.5. The graph shows the percentage of PH3+ cells among TBR2+ and GFP+ cells. Arrows indicate GFP+, PH3+, and TBR2+ cells. Data were presented as the mean ± SD (N = 3 mice, six sections). NS, not significant, unpaired Student’s t-test (A, B) and one-way ANOVA with Turkey’s post hoc test (C). Scale bars, 20 µm (A, C) and 50 µm (B). Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39815005), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of ROBO1 by Immunohistochemistry

Constitutive knock-out analysis indicates a role for Slit2, Slit3, and Robo1 during cardiac innervation development.(A) Representative images of Robo1+/+ and Robo1-/- sections stained by immunohistochemistry for the myocardium using cardiac Troponin I (cTnI) in purple and total innervation using Peripherin (Prph) in green. Arrowhead points to reduced total innervation staining in green in the Robo1-/-. (B) Quantification of total ventricular innervation corrected for ventricle volume (myocardial marker cTnI) in constitutive knock-outs and wild-type controls. T-test or one-way ANOVA with Tukey’s test per mouse line. (C) Quantification of total ventricular volume based on myocardial marker cTnI in constitutive knock-outs and wild-type controls. T-test or one-way ANOVA with Tukey’s test per mouse line. Scale bars, 100 μm.Figure 1—source data 1.Source data Figure 1B - total innervation measurements.Figure 1—source data 2.Source data Figure 1C - ventricle size measurements.Source data Figure 1B - total innervation measurements.Source data Figure 1C - ventricle size measurements. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/105932), licensed under a CC-BY license. Not internally tested by R&D Systems.