RBBP4/RbAp48 Antibody (24GB1240)

Novus Biologicals | Catalog # NBP3-51498

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB1240
View all RBBP4 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human RBBP4/RbAp48 (Uniprot: Q09028)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBBP4/RbAp48 Antibody (24GB1240)

RBBP4/RbAp48 Antibody (24GB1240)

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (24GB1240)[NBP3-51498] -

RBBP4/RbAp48 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: RBBP4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; RBBP4 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
RBBP4/RbAp48 Antibody (24GB1240)

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (24GB1240)[NBP3-51498] -

Immunocytochemical staining of HepG2 cells with RBBP4/RbAp48 antibody (1:1,000). Nuclei were stained blue with DAPI; RBBP4/RbAp48 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
RBBP4/RbAp48 Antibody (24GB1240)

Flow Cytometry: RBBP4/RbAp48 Antibody (24GB1240)[NBP3-51498] -

Validation of RBBP4/RbAp48 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with RBBP4/RbAp48 antibody (1:2,000) and analyzed using BD flow cytometer.
RBBP4/RbAp48 Antibody (24GB1240)

Flow Cytometry: RBBP4/RbAp48 Antibody (24GB1240)[NBP3-51498] -

Flow cytometric analysis of RBBP4/RbAp48expression in HepG2 cells using RBBP4/RbAp48 antibody (1:2,000). Green, isotype control; red, RBBP4.
RBBP4/RbAp48 Antibody (24GB1240)

Western Blot: RBBP4/RbAp48 Antibody (24GB1240)[NBP3-51498] -

Western blotting analysis using RBBP4/RbAp48 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for RBBP4/RbAp48 Antibody (24GB1240)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RBBP4

RbAp48 encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.

Long Name

Retinoblastoma Binding Protein 4

Alternate Names

CAF-1 p48, CAF-1C, NURF55, RBAP48

Gene Symbol

RBBP4

Additional RBBP4 Products

Product Documents for RBBP4/RbAp48 Antibody (24GB1240)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RBBP4/RbAp48 Antibody (24GB1240)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RBBP4/RbAp48 Antibody (24GB1240)

There are currently no reviews for this product. Be the first to review RBBP4/RbAp48 Antibody (24GB1240) and earn rewards!

Have you used RBBP4/RbAp48 Antibody (24GB1240)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies