RelB Antibody

Name: RelB Antibody
Brand: Novus Biologicals
SKU: NBP2-20123
Price: 469 USD
Availability: InStock
Rating: 5 (1 reviews)

Novus Biologicals | Catalog # NBP2-20123

(1)

Citations (1)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all RelB Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RelB. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RelB Antibody

Western Blot: RelB Antibody [NBP2-20123]

Western Blot: Rel B Antibody [NBP2-20123] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:10000.

Western Blot: Rabbit Polyclonal RelB Antibody [NBP2-20123] -

Western Blot: Rabbit Polyclonal RelB Antibody [NBP2-20123] - Whole cell lysates from MDA-MB-231, SUM159, MCF-7 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. RRS1 Antibody (NBP2-49323) was used for primary antibody: 1:1000, 4C, overnight. Image from a verified customer review.

Western Blot: RelB Antibody [NBP2-20123] -

Western Blot: RelB Antibody [NBP2-20123] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody (NBP2-20123) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: RelB Antibody [NBP2-20123] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody (NBP2-20123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunoprecipitation: RelB Antibody [NBP2-20123] -

RelB antibody immunoprecipitates RelB protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of RelB protein by 2 ug of RelB antibody (NBP2-20123) 7.5% SDS-PAGE The immunoprecipitated RelB protein was detected by RelB antibody (NBP2-20123) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Immunohistochemistry-Paraffin: RelB Antibody [NBP2-20123] -

RelB antibody detects RelB protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RelB stained by RelB antibody (NBP2-20123) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Chromatin Immunoprecipitation: RelB Antibody [NBP2-20123] -

Cross-linked ChIP was performed with THP-1 chromatin extract treated with LPS (1.0 ug/ml for 3 h) and 5 ug of either normal rabbit IgG or anti-RelB antibody. The precipitated DNA was detected by PCR with primer set targeting to IL-1betapromoter.

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Applications for RelB Antibody

Application

Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000

Application Notes

Chip assay-Assay dependent

Reviewed Applications

Read 1 review rated 5 using NBP2-20123 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RelB

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog B

Alternate Names

IREL

Gene Symbol

RELB

Additional RelB Products

Product Documents for RelB Antibody

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RelB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Transcription Factors and Regulators

C-type Lectin Receptors

Dendritic Cell Development

IL-1 Family

Interferons

NFkB Pathway

NOD-like Receptors and the Inflammasome

PDGF Family

RIG-I-like Receptors

Toll-Like Receptors

Transcription Factors in the Akt Pathway

Citations for RelB Antibody

Customer Reviews for RelB Antibody (1)

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RelB Antibody

Name: Anonymous

Application: Western Blot

Verified Customer | Posted 12/31/2023

Western Blot: whole cell lysates from MDA-MB-231, SUM159, MCF-7 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. RRS1 Antibody (NBP2-49323) was used for primary antibody: 1:1000, 4℃, overnight.