Rictor Antibody

Immunocytochemistry/ Immunofluorescence: Rictor Antibody [NB100-1534] -

Immunocytochemistry/ Immunofluorescence: Rictor Antibody [NB100-1534] - The combined silencing of Rictor & EGFR in vivo results in a complete inhibition of tumor growth.U251Ng2x, U251Rictor, U251EGFR & U251EGFR/Rictor cells were implanted into the right caudate nucleus-putamen of Rag2M mice (n = 6−8). Induction of shRNA expression in mice was initiated on day 21 by dissolving 2 mg/mL doxycyline & 5% sucrose in drinking water. a) On day 49, animals were imaged by Maestro™ fluorescence imaging unit for the expression of tRFP co-expressed with the shRNA sequences upon doxycycline-induced expression. Mice were then terminated & brains were harvested, sectioned & stained for nuclei, Rictor, EGFR & p(473)-AKT & imaged for all markers in addition to tRFP by robotic fluorescence microscopy. No tumor was detected in the U251EGFR/Rictor group. b) A representative brain section from U251Ng2x, U251Rictor, U251EGFR & U251EGFR/Rictor tumor groups is shown: tRFP (red) & Hoechst (blue). c) A representative tumor section from U251Ng2x, U251Rictor & U251EGFR tumor groups is shown: nuclei (blue), rRFP (red), Rictor (yellow), EGFR (green) & p(473)-AKT (orange). d) The expression of EGFR (left axis), Rictor (right axis) & p(473)-AKT (right axis) in U251Ng2x, U251Rictor, U251EGFR tumor sections were quantified (positive staining normalized to Hoechst nuclei staining). e) Tumor sizes were estimated by quantification of tumor areas in brain sections from all groups (left axis). The expression of the proliferation marker Ki67 in the tumor (proliferating fraction) was also quantified (right axis). *p-value ≤0.05; **p-value ≤0.01; ***p-value ≤0.001 compared to control untreated cells. ‡: No tumor was detected in the U251EGFR/Rictor group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23555046), licensed under a CC-BY license. Not internally tested by Novus Biologicals.