RING1 Antibody (23GB955)

Novus Biologicals | Catalog # NBP3-52005

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB955
View all RING1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human RING1 (Uniprot: Q06587)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RING1 Antibody (23GB955)

RING1 Antibody (23GB955)

Immunocytochemistry/ Immunofluorescence: RING1 Antibody (23GB955)[NBP3-52005] -

RING1 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: RING1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; RING1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
RING1 Antibody (23GB955)

Immunocytochemistry/ Immunofluorescence: RING1 Antibody (23GB955)[NBP3-52005] -

Immunocytochemical staining of HepG2 cells with RING1 antibody (1:1,000). Nuclei were stained blue with DAPI; RING1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
RING1 Antibody (23GB955)

Flow Cytometry: RING1 Antibody (23GB955)[NBP3-52005] -

Validation of RING1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with RING1 antibody (1:2,000) and analyzed using BD flow cytometer.
RING1 Antibody (23GB955)

Flow Cytometry: RING1 Antibody (23GB955)[NBP3-52005] -

Flow cytometric analysis of RING1expression in HepG2 cells using RING1 antibody (1:2,000). Green, isotype control; red, RING1.

Applications for RING1 Antibody (23GB955)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RING1

RING1 belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq]

Long Name

E3 ubiquitin-protein ligase RING1

Alternate Names

RNF1

Gene Symbol

RING1

Additional RING1 Products

Product Documents for RING1 Antibody (23GB955)

Certificate of Analysis

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Product Specific Notices for RING1 Antibody (23GB955)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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