RNA Polymerase II/POLR2A Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-32080
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Cited:
Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Chemotaxis
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 754 to 804 of human RNA Polymerase II using the numbering given in entry NP_000928.1 (GeneID 5430).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNA Polymerase II/POLR2A Antibody - BSA Free
Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A Antibody [NBP2-32080] - ChIP-qPCR analysis of RNA polymerase II binding at pause site of the Jun B gene. Chromatin isolated from one 15 cm plate of HeLa cells was immunoprecipitated with 8.0 ug of anti-RNA polymerase II antibodies and PCR amplified.
Immunoprecipitation: Rabbit Polyclonal RNA Polymerase II/POLR2A Antibody [NBP2-32080] -
Immunoprecipitation: Rabbit Polyclonal RNA Polymerase II/POLR2A Antibody [NBP2-32080] - Detection of human RNA Polymerase II by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNA Polymerase II antibody (NBP2-32080 lot 2) used for IP at 6 ug per reaction. RNA Polymerase II was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of RNA Polymerase II antibody. For blotting immunoprecipitated RNA Polymerase II, the second antibody was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.Applications for RNA Polymerase II/POLR2A Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
8 µg per IP
Immunoprecipitation
2 - 10 ug/mg lysate
Reviewed Applications
Read 1 review rated 5 using NBP2-32080 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RNA Polymerase II/POLR2A
Long Name
DNA-directed RNA Polymerase II Subunit RPB1
Alternate Names
POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2
Gene Symbol
POLR2A
UniProt
Additional RNA Polymerase II/POLR2A Products
Product Documents for RNA Polymerase II/POLR2A Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RNA Polymerase II/POLR2A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for RNA Polymerase II/POLR2A Antibody - BSA Free
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Application: Western Blot and ImmunoprecipitationSample Tested: Hep3B human hepatocellular carcinoma cell lineSpecies: HumanVerified Customer | Posted 05/01/2024Hep3B cells were lysed in immunoprecipitation lysis buffer and incubated 20 min on ice. Following centrifugation at 18,000 x g at 4C, lysates were precleared with protein G-Sepharose for 2 h with rotation. 1 mg of lysate was incubated with rotation overnight at 4C with 2 ug of NBP2-32080 antibody, followed by incubation with protein G-Sepharose for 2 h at 4C and washes with IP lysis buffer 4x for 5 min at 4C. Immunoprecipitates were used for immunoblot assay. WB was performed using a 1:1000 dilution of RNA Pol II antibody in 1% BSA/PBS.
Bio-Techne ResponseThis review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
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Protocols
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