RNF2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-49966
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Species Reactivity
Validated:
Cited:
Predicted:
Applications
Validated:
Cited:
Label
Antibody Source
Format
Product Specifications
Immunogen
Clonality
Host
Isotype
Scientific Data Images for RNF2 Antibody - BSA Free
Western Blot: RNF2 Antibody [NBP1-49966]
Western Blot: RNF2 Antibody [NBP1-49966] - Detection of Human and Mouse RNF2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from K562 (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RNF2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RNF2 was also immunoprecipitated by rabbit anti-RNF2 antibody, which recognizes a downstream epitope. For blotting immunoprecipitated RNF2 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-49966]
Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-49966] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RNF2 used at a dilution of 1:1,000 (1ug/ml). Detection: Vector ImmPACT NovaREDImmunohistochemistry: RNF2 Antibody [NBP1-49966]
RNF2-Antibody-Immunohistochemistry-NBP1-49966-img0004.jpgImmunohistochemistry: RNF2 Antibody [NBP1-49966] -
Immunohistochemistry: RNF2 Antibody [NBP1-49966] - Epigenetic remodelers were overexpressed in pancreatitis & pancreatic cancer(A) Immunohistochemistry staining of Bmi1, Ring1b & H2AK119ub in eight week old control mice, 48 hours & seven days after the last cerulein injection (n = 5). Representative images include boxes with high magnification to demonstrate nuclear staining of centroacinar (control) & ADM (48h) cells. Bar, 100 μm. (B) Positive stained nuclei of Bmi1, Ring1b & H2AK119ub per high power field (HPF) were counted & quantified. Data are presented as mean ± SEM from three pictures of each animal. (C) Immunohistochemistry staining against Bmi1, Ring1b & H2AK119ub of pancreatic tissue from three week old KPC mice representing morphological normal tissue, from ten week old mice showing preinvasive ductal lesions & from 20 week old KPC mice demonstrating full PDAC (n = 5). High magnification images represent nuclear staining of centroacinar cells (3w-normal) or ADMs (10w-ADM). Bar, 100 μm. (D) Positive stained nuclei of Bmi1, Ring1b & H2AK119ub per high power field (HPF) were counted & quantified. Data are presented as mean ± SEM from three pictures of each animal. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26716510), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for RNF2 Antibody - BSA Free
Immunohistochemistry
Immunohistochemistry-Paraffin
Immunoprecipitation
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: RNF2
Long Name
Alternate Names
Gene Symbol
UniProt
Additional RNF2 Products
Product Documents for RNF2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RNF2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for RNF2 Antibody - BSA Free
Customer Reviews for RNF2 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review RNF2 Antibody - BSA Free and earn rewards!
Have you used RNF2 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars