ROCK2 Antibody (23GB2030)

Name: ROCK2 Antibody (23GB2030)
Brand: Novus Biologicals
SKU: NBP3-50978
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-50978

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2030

View all ROCK2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human ROCK2 (Uniprot: O75116)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROCK2 Antibody (23GB2030)

Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (23GB2030)[NBP3-50978] -

ROCK2 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: ROCK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ROCK2 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.

Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (23GB2030)[NBP3-50978] -

Immunocytochemical staining of HepG2 cells with ROCK2 antibody (1:1,000). Nuclei were stained blue with DAPI; ROCK2 cochaperone 3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.

Flow Cytometry: ROCK2 Antibody (23GB2030)[NBP3-50978] -

Validation of ROCK2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ROCK2 antibody (1:2,000) and analyzed using BD flow cytometer.

Flow Cytometry: ROCK2 Antibody (23GB2030)[NBP3-50978] -

Flow cytometric analysis of ROCK2expression in HepG2 cells using ROCK2 antibody (1:2,000). Green, isotype control; red, ROCK2.

Western Blot: ROCK2 Antibody (23GB2030)[NBP3-50978] -

Western blotting analysis using ROCK2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Western Blot: ROCK2 Antibody (23GB2030)[NBP3-50978] -

Western blotting analysis using ROCK2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

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Applications for ROCK2 Antibody (23GB2030)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ROCK2

ROCK2 is encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Long Name

Rho-associated, Coiled-coil Containing Protein Kinase 2

Alternate Names

ROCK-II, ROKalpha

Gene Symbol

ROCK2

Additional ROCK2 Products

Product Documents for ROCK2 Antibody (23GB2030)

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Certificate of Analysis

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Product Specific Notices for ROCK2 Antibody (23GB2030)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.