![Western Blot: RPSA Antibody (MLuC5) [NB200-631]](https://resources.rndsystems.com/images/products/RPSA-Antibody-MLuC5-Western-Blot-NB200-631-img0005.jpg "Western Blot: RPSA Antibody (MLuC5) [NB200-631]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # MLuC5
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Product Specifications
Immunogen
Small cell lung carcinoma N592 live cells
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 21320497).
Localization
Cell membrane with some cytoplasmic.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for RPSA Antibody (MLuC5)
Western Blot: RPSA Antibody (MLuC5) [NB200-631]
Western Blot: RPSA Antibody (MLuC5) [NB200-631] - Analysis of 25 ug of HepG2 (lane 1) and Hela (lane 2) cell lysates.![Immunocytochemistry/ Immunofluorescence: RPSA Antibody (MLuC5) [NB200-631]](https://resources.rndsystems.com/images/products/RPSA-Antibody-MLuC5-Immunocytochemistry-Immunofluorescence-NB200-631-img0004.jpg "Immunocytochemistry/ Immunofluorescence: RPSA Antibody (MLuC5) [NB200-631]")
Immunocytochemistry/ Immunofluorescence: RPSA Antibody (MLuC5) [NB200-631]
Immunocytochemistry/Immunofluorescence: RPSA Antibody (MLuC5) [NB200-631] - Analysis of Laminin Receptor (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left).![Immunohistochemistry-Paraffin: RPSA Antibody (MLuC5) [NB200-631]](https://resources.rndsystems.com/images/products/RPSA-Antibody-MLuC5-Immunohistochemistry-Paraffin-NB200-631-img0002.jpg "Immunohistochemistry-Paraffin: RPSA Antibody (MLuC5) [NB200-631]")
Immunohistochemistry-Paraffin: RPSA Antibody (MLuC5) [NB200-631]
Immunohistochemistry-Paraffin: RPSA Antibody (MLuC5) [NB200-631] - Human breast carcinoma stained with Laminin Receptor antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane with some cytoplasmic staining of tumor cells.![Flow Cytometry: RPSA Antibody (MLuC5) [NB200-631]](https://resources.rndsystems.com/images/products/RPSA-Antibody-MLuC5-Flow-Cytometry-NB200-631-img0003.jpg "Flow Cytometry: RPSA Antibody (MLuC5) [NB200-631]")
Flow Cytometry: RPSA Antibody (MLuC5) [NB200-631]
Flow Cytometry: RPSA Antibody (MLuC5) [NB200-631] - Analysis of Laminin Receptor in MCF-7 cells (green) compared to an isotype control (blue).Applications for RPSA Antibody (MLuC5)
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:200
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
4 - 8 ug/mL
Western Blot
1:10 - 1:200
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RPSA
Alternate Names
37 kDa laminin receptor precursor, 37/67 kDa laminin receptor, 37LRPNEM/1CHD4, 67 kDa laminin receptor, 67kD, ribosomal protein SA, 67LR, Colon carcinoma laminin-binding protein, LAMBR37 kDa laminin receptor, laminin binding protein, Laminin receptor 1, laminin receptor 1 (67kD, ribosomal protein SA), Laminin-binding protein precursor p40, LamR, LAMR 1, LAMR140S ribosomal protein SA, LBP, LBP/p40, LRP, LRP/LR, Multidrug resistance-associated protein MGr1-Ag, p40, ribosomal protein SA
Entrez Gene IDs
3921 (Human)
Gene Symbol
RPSA
UniProt
Additional RPSA Products
Product Documents for RPSA Antibody (MLuC5)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RPSA Antibody (MLuC5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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