RSK2 [p Ser227] Antibody

Novus Biologicals | Catalog # NBP3-13244

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RSK2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser227 of human RSK2. The exact sequence is proprietary.

Modification

p Ser 227

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for RSK2 [p Ser227] Antibody

Western Blot: RSK2 [p Ser227] Antibody [NBP3-13244]

Western Blot: RSK2 [p Ser227] Antibody [NBP3-13244]

Western Blot: RSK2 [p Ser227] Antibody [NBP3-13244] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK2 (phospho Ser227) antibody (NBP3-13244) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: RSK2 [p Ser227] Antibody [NBP3-13244]

Western Blot: RSK2 [p Ser227] Antibody [NBP3-13244]

Western Blot: RSK2 [p Ser227] Antibody [NBP3-13244] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RSK2 (phospho Ser227) antibody (NBP3-13244) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for RSK2 [p Ser227] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RSK2

Ribosomal protein S6 kinase II (RSK2) is a protein serine/threonine kinase that was originally detected by its ability to catalyze the multi-site phosphorylation of the 40S ribosomal protein S6 in vitro. One of the important substrates of RSK may be the Glycogenbinding subunit of Protein phosphatase-1, which when phosphorylated by RSK, appears to activate Protein phosphatase 1. This results in increased dephosphorylation and activation of Glycogen synthase to enhance Glycogen synthesis.

Long Name

Ribosomal Protein S6 Kinase II

Alternate Names

ISPK-1, pp90RSK2, RPS6KA3

Gene Symbol

RPS6KA3

Additional RSK2 Products

Product Documents for RSK2 [p Ser227] Antibody

Certificate of Analysis

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Product Specific Notices for RSK2 [p Ser227] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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