RUNX1/CBFA2 Antibody (SD0803)
Novus Biologicals | Catalog # NBP2-67779
Recombinant Monoclonal Antibody
![Western Blot: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]](https://resources.rndsystems.com/images/products/RUNX1-CBFA2-Antibody-SD0803-Western-Blot-NBP2-67779-img0006.jpg "Western Blot: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SD0803 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human RUNX1/CBFA2 aa 404-453 / 453. (SwissProt: Q01196 Human; SwissProt: Q13761 Human; SwissProt: Q13950 Human; SwissProt: Q03347 Mouse; SwissProt: Q08775 Mouse; SwissProt: Q64131 Mouse; SwissProt: Q63046 Rat; SwissProt: Q9Z2J9 Rat)
Localization
Nucleus, Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RUNX1/CBFA2 Antibody (SD0803)
Western Blot: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]
Western Blot: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of RUNX1+RUNX2+RUNX3 on Jurkat cells lysates using anti-RUNX1+RUNX2+RUNX3 antibody at 1/1,000 dilution.![Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]](https://resources.rndsystems.com/images/products/RUNX1-CBFA2-Antibody-SD0803-Immunocytochemistry-Immunofluorescence-NBP2-67779-img0007.jpg "Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]")
Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]
Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Staining RUNX1+RUNX2+RUNX3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]](https://resources.rndsystems.com/images/products/RUNX1-CBFA2-Antibody-SD0803-Immunohistochemistry-Paraffin-NBP2-67779-img0005.jpg "Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]")
Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]
Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of paraffin-embedded mouse testis tissue using anti-RUNX1+RUNX2+RUNX3 antibody. Counter stained with hematoxylin.![Flow Cytometry: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]](https://resources.rndsystems.com/images/products/RUNX1-CBFA2-Antibody-SD0803-Flow-Cytometry-NBP2-67779-img0001.jpg "Flow Cytometry: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]")
Flow Cytometry: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]
Flow Cytometry: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of Jurkat cells with RUNX1+RUNX2+RUNX3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody![Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]](https://resources.rndsystems.com/images/products/RUNX1-CBFA2-Antibody-SD0803-Immunocytochemistry-Immunofluorescence-NBP2-67779-img0002.jpg "Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]")
Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]
Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Staining RUNX1+RUNX2+RUNX3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]](https://resources.rndsystems.com/images/products/RUNX1-CBFA2-Antibody-SD0803-Immunocytochemistry-Immunofluorescence-NBP2-67779-img0003.jpg "Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]")
Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]
Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Staining RUNX1+RUNX2+RUNX3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]](https://resources.rndsystems.com/images/products/RUNX1-CBFA2-Antibody-SD0803-Immunohistochemistry-Paraffin-NBP2-67779-img0004.jpg "Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]")
Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779]
Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX1+RUNX2+RUNX3 antibody. Counter stained with hematoxylin.Applications for RUNX1/CBFA2 Antibody (SD0803)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RUNX1/CBFA2
Long Name
Runt-related Transcription Factor 1
Alternate Names
AML1, CBFA2
Gene Symbol
RUNX1
Additional RUNX1/CBFA2 Products
Product Documents for RUNX1/CBFA2 Antibody (SD0803)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RUNX1/CBFA2 Antibody (SD0803)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
