S100A/B Antibody

Novus Biologicals | Catalog # NB200-538

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all S100A/B Primary Antibodies »

Product Specifications

Immunogen

Affinity purified S-100 protein isolated from cow brain.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:31633020).

Localization

Cytoplasmic

Specificity

This antibody reacts with cow S-100 protein a and b. It shows strong crossreactivity with human S-100 a and b. In the brain, it labels glial and ependymal cells. Melanocytes and Langerhans cells of the skin stain positive with this antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for S100A/B Antibody

Immunohistological Staining of S100A/B in Paraffin Embedded Human Skin

Immunohistological Staining of S100A/B in Paraffin Embedded Human Skin

Formalin fixed paraffin embedded human skin stained with S100 antibody (NB200-538).

Applications for S100A/B Antibody

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:100
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Proprietary Formulation

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: S100A/B

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein may function in stimulation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. Immunoreactive S100 protein localizes in the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. Ganglion cells do not stain for S100 protein. S100 can be detected in almost all benign naevi and malignant melanocytic tumors of the skin, and in Langerhans cells in the skin.

Alternate Names

protein S100-A1, S100 alpha, S100 calcium binding protein A1, S100 calcium-binding protein A1S100, S-100 protein alpha chain, S-100 protein subunit alpha, S100 protein, alpha polypeptide, S100A, S100-alpha

Entrez Gene IDs

6285 (Human); 6271 (Human); 20193 (Mouse); 528735 (Bovine)

Gene Symbol

S100B

UniProt

P23297, P56565 (Mouse)

Additional S100A/B Products

Product Documents for S100A/B Antibody

Certificate of Analysis

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Product Specific Notices for S100A/B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for S100A/B Antibody

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Protocols

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