SARS-CoV NSP8 Antibody (5A10) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13528
![Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]](https://resources.rndsystems.com/images/products/SARS-CoV-NSP8-Antibody-5A10-Western-Blot-NBP3-13528-img0002.jpg "Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]")
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Key Product Details
Species Reactivity
SARS-CoV, SARS-CoV-2
Applications
Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5A10
Format
Azide and BSA Free
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Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to SARS virus nsp8.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Centrifuge briefly prior to opening.
Scientific Data Images for SARS-CoV NSP8 Antibody (5A10) - Azide and BSA Free
Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]
Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - Non-infected (-) and infected (+, 48h pl MOI 0.01) whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]](https://resources.rndsystems.com/images/products/SARS-CoV-NSP8-Antibody-5A10-Immunocytochemistry-Immunofluorescence-NBP3-13528-img0003.jpg "Immunocytochemistry/ Immunofluorescence: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]")
Immunocytochemistry/ Immunofluorescence: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]
Immunocytochemistry/Immunofluorescence: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] detects SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 stained by SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) diluted at 1:1000. Blue: Fluoroshield with DAPI.![Flow Cytometry: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]](https://resources.rndsystems.com/images/products/SARS-CoV-NSP8-Antibody-5A10-Flow-Cytometry-NBP3-13528-img0004.jpg "Flow Cytometry: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]")
Flow Cytometry: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]
Flow Cytometry: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis. Sample: Vero E6 cells infected with SARS-CoV-2. Black: Uninfected Vero E6 cells was used as a control. Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) dilution: 1:100.![Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]](https://resources.rndsystems.com/images/products/SARS-CoV-NSP8-Antibody-5A10-Western-Blot-NBP3-13528-img0001.jpg "Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]")
Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528]
Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) diluted at 1:5000.Applications for SARS-CoV NSP8 Antibody (5A10) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Assay dependent
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry-Frozen
Assay dependent
Western Blot
1:1000-1:10000
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NSP8
Long Name
Non-Structural Protein 8
Alternate Names
COVID-19
Additional NSP8 Products
Product Documents for SARS-CoV NSP8 Antibody (5A10) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SARS-CoV NSP8 Antibody (5A10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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