SARS Nucleocapsid Protein Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56576

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

SARS-CoV, SARS-CoV-2, Virus

Cited:

Human, Hamster, SARS-CoV, Viral, Virus, Virus - SARS-CoV-2

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, SDS-PAGE

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, IF/ICC, IF/IHC, In Situ Hybridization, Mass Spectrometry, RNAscope compatible, SDS-PAGE

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SARS Nucleocapsid Protein Primary Antibodies »

Product Specifications

Immunogen

The antibody was developed by immunizing Rabbit with a synthetic peptide corresponding to amino acids 399-411 (DLDDFSKQLQQSM-C) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)

Reactivity Notes

Use in SARS-CoV-2 reported in scientific literature (PMID:33807059).

Specificity

Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Nucleocapsid protein (NBP2-90975) and low/no reactivity towards H1N1 (NBP1-99041). No cross-reactivity observed with influenza A(H1N1) virus, influenza B virus, respiratory syncytial virus, parainfluenza virus type 3, human coronavirus (HCoV) 229E, or MERS-CoV in PCR-confirmed tissue samples [PMID:32437316].

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS Nucleocapsid Protein Antibody - BSA Free

Immunohistochemistry: SARS Nucleocapsid Protein Antibody - BSA Free [NB100-56576]

Immunohistochemistry: SARS Nucleocapsid Protein Antibody - BSA Free [NB100-56576]

Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): scattered immunostaining of tracheal epithelial cells. B) P5: higher magnification shows immunostaining of ciliated cells. C) P8: immunostaining of desquamated type I pneumocyte in an alveolar lumen. D) P4: colocalization of SARS-CoV-2 viral antigen (red) with type II pneumocyte stained by surfactant (brown; arrow). E) P4: colocalization of SARS-CoV-2 viral antigen (red) with macrophages stained by CD163 (brown; arrows); virus immunostaining within type II pneumocytes is also seen (arrowheads). F) P4: extensive immunostaining of hyaline membranes in a region of exudative DAD. G) P3: scattered immunostaining within macrophage in hilar lymph node; anthracosis is also present. Emerg Infect Dis. 2020 May 21;26(9) 10.3201/eid2609.202095, PMID: 32437316
Immunohistochemistry: SARS Nucleocapsid Protein Antibody - BSA Free [NB100-56576]

Immunohistochemistry: SARS Nucleocapsid Protein Antibody - BSA Free [NB100-56576]

Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. Magnification 400x. (j) SARS-CoV-2 antigen is detected by immunohistochemistry in type I pneumocytes (asterisk) and type II pneumocytes (arrow) as well as alveolar macrophages (arrowheads). Magnification 400x. (k) SARS-CoV-2 antigen is detected by immunohistochemistry in mediastinal lymph node. Magnification 400x. (l) SARSCoV-2 antigen is detected by immunohistochemistry in macrophages and lymphocytes in the lamina propria of the cecum. Magnification 400x. bioRxiv March 21, 2020 https://doi.org/10.1101/2020.03.21.001628
Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56576] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S (848561); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.
Immunohistochemistry: SARS Nucleocapsid Protein Antibody - BSA Free [NB100-56576]

Immunohistochemistry: SARS Nucleocapsid Protein Antibody - BSA Free [NB100-56576]

SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0003.jpg
Western Blot: SARS Nucleocapsid Protein AntibodyBSA Free [NB100-56576]

Western Blot: SARS Nucleocapsid Protein AntibodyBSA Free [NB100-56576]

Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - Western blot shows recombinant SARS-CoV-2 Nucleocapsid protein. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-SARS-CoV-2 Nucleocapsid Polyclonal Antibody (Catalog # NB100-56576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for SARS-CoV-2 Nucleocapsid at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1
Western Blot: SARS Nucleocapsid Protein AntibodyBSA Free [NB100-56576]

Western Blot: SARS Nucleocapsid Protein AntibodyBSA Free [NB100-56576]

Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody.
SARS Nucleocapsid Protein Antibody - BSA Free

Immunohistochemistry-Frozen: Rabbit Polyclonal SARS Nucleocapsid Protein Antibody [NB100-56576]

Immunohistochemistry-Frozen: Rabbit Polyclonal SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of mouse adult lung infected with SARS-CoV-2. Image from a verified customer review.

Applications for SARS Nucleocapsid Protein Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (Mossel et al (2008))

Immunohistochemistry

reported in scientific literature (PMID 32396922)

Immunohistochemistry-Frozen

Validated for Immunohistochemistry-Frozen from a verified customer review.

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24725942)

SDS-PAGE

reported in scientific literature (PMID 34880383)

Western Blot

1:100-1:2000

Reviewed Applications

Read 1 review rated 4 using NB100-56576 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS Nucleocapsid Protein

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Alternate Names

N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome, 2019-nCoV Nucleocapsid Protein, COVID-19 N protein, SARS-CoV-2 N protein

Gene Symbol

N

Additional SARS Nucleocapsid Protein Products

Product Documents for SARS Nucleocapsid Protein Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SARS Nucleocapsid Protein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SARS Nucleocapsid Protein Antibody - BSA Free

Customer Reviews for SARS Nucleocapsid Protein Antibody - BSA Free (1)

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Customer Images

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  • SARS Nucleocapsid Protein Antibody - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adult lung
    Species: Mouse
    Verified Customer | Posted 04/09/2025
    Immunostaining of Lung infected with SARS-CoV-2
    SARS Nucleocapsid Protein Antibody - BSA Free NB100-56576

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Protocols

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FAQs for SARS Nucleocapsid Protein Antibody - BSA Free

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  • Q: does your antibody NB100-56576 recognizes SARS-CoV-2 or only SARS-Cov? (Image 1 indicates Cov2 in the legend, but the data sheet only states SARS coronavirus

    A:

    This antibody was originally developed against SARS, but due to identity to SARS-CoV-2 was predicted to cross-react. the image in question (#1) is from a recent publication that used NB100-56576 to detect SARS-CoV-2 nucleocapsid. Here is the link to the publication if you would like to take a look https://www.biorxiv.org/content/10.1101/2020.03.21.001628v1

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