SARS Nucleocapsid Protein Antibody - BSA Free

This SARS Nucleocapsid Protein Antibody was developed by immunizing rabbits with a full-length recombinant protein from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Uniprot#: P59595)

SARS Coronavirus. Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (91%)

The product was purified from monospecific antiserum by protein A affinity purification.

Polyclonal

Rabbit

IgG

The recombinant nucleocapsid protein was prepared as follows: SUMO-Nucleocapsid fusion was expressed in E.coli in LB medium and purified using Ni-NTA resin (Qiagen) affinity chromatography. After the fusion was cleaved by the SUMO Protease, the SUMO tag and protease were subtracted from the nucleocapsid using MAC and the nucleocapsid was finally purified using Cation Exchange Chromatography with the Macro-Prep High S resin (BioRad) and size exclusion chromatography.
NBP2-44205 is manufactured exclusively by Rockland Immunochemicals.

This protein A purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein. The product was purified from monospecific antiserum by protein A affinity purification

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Protein A purified

For the 500 ug size, we recommend reconstituting with 100 ul deionized water. The 0.1 mg size is provided in liquid format (0.02 ml).

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

BSA Free

0.01% Sodium Azide

LYOPH mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.