SCYL2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-93622

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SCYL2 (NP_060458.3). MESMLNKLKSTVTKVTADVTSAVMGNPVTREFDVGRHIASGGNGLAWKIFNGTKKSTKQEVAVFVFDKKLIDKYQKFEKDQIIDSLKRGVQQLTRLRHPRLLTVQHPLEESRDCLAFCTEPVFASLANVLGNWENLPSPISPDIKDYKLYDVETKYGLLQVSEGLSFLHSSVKMVHGNITPENIILNKSGAWKIMGFDFCVSSTNPSEQEPKFPCKEWDPNLPSLCLPNPEYLAPEYILSVSCETASDMYSLGTVMYAVFNKGKPIFEVNKQDIYKSFSRQLDQLSRLGSSSLTNIPEEV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SCYL2 Antibody - Azide and BSA Free

Western Blot: SCYL2 AntibodyAzide and BSA Free [NBP2-93622]

Western Blot: SCYL2 AntibodyAzide and BSA Free [NBP2-93622]

Western Blot: SCYL2 Antibody [NBP2-93622] - Analysis of extracts of various cell lines, using SCYL2 at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: SCYL2 Antibody - Azide and BSA Free [NBP2-93622]

Immunocytochemistry/ Immunofluorescence: SCYL2 Antibody - Azide and BSA Free [NBP2-93622]

Immunocytochemistry/Immunofluorescence: SCYL2 Antibody [NBP2-93622] - Immunofluorescence analysis of U2OS cells using SCYL2 Rabbit pAb (NBP2-93622) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: SCYL2 Antibody - Azide and BSA Free [NBP2-93622]

Immunocytochemistry/ Immunofluorescence: SCYL2 Antibody - Azide and BSA Free [NBP2-93622]

Immunocytochemistry/Immunofluorescence: SCYL2 Antibody [NBP2-93622] - Immunofluorescence analysis of L929 cells using SCYL2 Rabbit pAb (NBP2-93622) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for SCYL2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SCYL2

SCYL2 is a component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane a

Alternate Names

CVAK104, FLJ10074, KIAA1360Coated vesicle-associated kinase of 104 kDa, SCY1-like 2 (S. cerevisiae), SCY1-like protein 2

Gene Symbol

SCYL2

Additional SCYL2 Products

Product Documents for SCYL2 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SCYL2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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