SEC62 Antibody (PSH0-25)

Novus Biologicals | Catalog # NBP3-32939

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH0-25 expressed in HEK293
View all SEC62 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human SEC62 aa 1-50 / 399. (Uniprot: Q99442)

Localization

Endoplasmic reticulum membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SEC62 Antibody (PSH0-25)

SEC62 Antibody (PSH0-25) Western Blot: SEC62 Antibody (PSH0-25) [NBP3-32939]

Western Blot: SEC62 Antibody (PSH0-25) [NBP3-32939]

Western blot analysis on different lysates with NBP3-32939 at 1/5,000 dilution. Lane 1: Hela cell lysate (20 µg/Lane) Lane 2: Hela cell lysate (20 µg/Lane) Lane 3: K562 cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: 293T cell lysate (20 µg/Lane) Lane 7: Jurkat cell lysate (20 µg/Lane) Predicted band size: 46 kDa Observed band size: 56 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32939 at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
SEC62 Antibody (PSH0-25) Western Blot: SEC62 Antibody (PSH0-25) [NBP3-32939]

Western Blot: SEC62 Antibody (PSH0-25) [NBP3-32939]

Western blot analysis on different lysates with NBP3-32939 at 1/5,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: PC-12 cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Rat liver tissue lysate (40 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 56 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32939 at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Applications for SEC62 Antibody (PSH0-25)

Application
Recommended Usage

Western Blot

1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SEC62

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC63 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. (provided by RefSeq)

Alternate Names

Dtrp1, hTP-1, S.cerevisiae, homolog of, SEC62 homolog (S. cerevisiae), TLOC1, TP-1, translocation protein 1, translocation protein SEC62

Gene Symbol

SEC62

Additional SEC62 Products

Product Documents for SEC62 Antibody (PSH0-25)

Certificate of Analysis

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Product Specific Notices for SEC62 Antibody (PSH0-25)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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