Separase Antibody (XJ11-1B12) - BSA Free

Novus Biologicals | Catalog # NB100-439

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # XJ11-1B12

Format

BSA Free
View all Separase Primary Antibodies »

Product Specifications

Immunogen

Maltose-Binding Protein fusion of a C-terminal fragment of human Separase (residues 1866-1996). [UniProt# Q14674]

Localization

Nuclear and Centrosomal

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Separase Antibody (XJ11-1B12) - BSA Free

Western Blot: Separase Antibody (XJ11-1B12)BSA Free [NB100-439]

Western Blot: Separase Antibody (XJ11-1B12)BSA Free [NB100-439]

Western Blot: Separase Antibody (XJ11-1B12) [NB100-439] - Separase detected in 5C6 cell lysates using NB 100-439. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.

Applications for Separase Antibody (XJ11-1B12) - BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
This Separase (XJ11-1B12) antibody is useful for Western Blot, where a band is seen at ~220 kDa (full-length form) and ~65 kDa (auto-processed form).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

0.9 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Separase

In normal cellular proliferation, the maintenance of constant centriole numbers is strongly associated with cell cycle process and the disengagement of mother-daugther centriole (a requirement for duplication of centriole) is facilitated by separase (ESPL1, a cysteine endopeptidase) mediated proteolytic cleavage of cohesin, a "glue" protein complex which is also essential to sister-chromatide cohesion. Separase is a caspase-like protease which plays a key role in chromosome segregation by cleaving SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle phases, separase is kept inactivated, and its proteolytic activity is tightly regulated by multiple inhibitory mechanisms combining securin binding, CyclinB1/Cdk1 mediated serine residue phosphorylation (pSer1226), PP2A binding and autocatalytic cleavage. Ectopic activation of separase proteolytic activity results in premature sister-chromatide separation/centriole disengagement and separase overexpression has been shown to induce aneuploidy and carcinogenesis.

Alternate Names

ESPL1, PRCE

Gene Symbol

ESPL1

Additional Separase Products

Product Documents for Separase Antibody (XJ11-1B12) - BSA Free

Certificate of Analysis

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Product Specific Notices for Separase Antibody (XJ11-1B12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

Citations for Separase Antibody (XJ11-1B12) - BSA Free

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Protocols

View specific protocols for Separase Antibody (XJ11-1B12) - BSA Free (NB100-439):

Separase Antibody (XJ11-1B12):
1. Load protein on gel (ie: ~15 ug of Ecdysone-inducible cell line 5C6) and run.
2. Transfer protein to nitrocellulose (Schleicher&Schuell, cat# BA83).
3. Block the membrane with 5% milk for 30 minutes at room temperature.
4. Incubate the membrane with anti-separase [cat# NB 100-439] (1:500), overnight at 4C or 2 h at room temperature. Milk or PBS-T are good for this dilution.
5. Wash the membrane 3 times, 10 minutes per wash in PBS with 0.1% Tween-20 (PBS-T).
6. Incubate with secondary antibody for 30 minutes at room temperature. Use milk in PBS-T as a diluent.
7. Rinse the membrane 2 times with deionized water and place in an ECL working solution.
8. Expose to the film.

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FAQs

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