SESN2 Antibody (3B8) - Azide and BSA Free

Novus Biologicals | Catalog # H00083667-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B8

Format

Azide and BSA Free
View all SESN2 Primary Antibodies »

Product Specifications

Immunogen

SESN2 (AAH13304.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIVADSECRAELKDYLRFAPGGVGDSGPGEEQRESRARRGPRGPSAFIPVEEVLREGAESLEQHLGLEALMSSGRVDNLAVVMGLHPDYFTSFWRLHYLLLHTDGPLASSWRHYIAIMAAARHQCSYLVGSHMAEFLQTGGDPEWLLGLHRAPEKLRKLSEINKLLAHRPWLITKEHIQALLKTGEHTWSLAELIQALVLLTHCHSLSSFVFGCGILPEGDADGSPAPQAPTPPSEQSSPPSRDPLNNSGGFESARDVEALMERMQQLQESLLRDEGTSQEEMESRFELEKSESLLVTPSADILEPSPHPDMLCFVEDPTFGYEDFTRRGAQAPPTFRAQDYTWEDHGYSLIQRLYPEGGQLLDEKFQAAYSLTYNTIAMHSGVDTSVLRRAIWNYIHCVFGIRYDDYDYGEVNQLLERNLKVYIKTVACYPEKTTRRMYNLFWRHFRHSEKVHVNLLLLEARMQAALLYALRAITRYMT

Reactivity Notes

Other species not tested.

Specificity

SESN2 - sestrin 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SESN2 Antibody (3B8) - Azide and BSA Free

Western Blot: SESN2 Antibody (3B8) [H00083667-M03]

Western Blot: SESN2 Antibody (3B8) [H00083667-M03]

Western Blot: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of SESN2 expression in transfected 293T cell line by SESN2 monoclonal antibody (M03), clone 3B8. Lane 1: SESN2 transfected lysatE (54.5 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: SESN2 Antibody (3B8) [H00083667-M03]

Immunocytochemistry/ Immunofluorescence: SESN2 Antibody (3B8) [H00083667-M03]

Immunocytochemistry/Immunofluorescence: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of monoclonal antibody to SES2 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: SESN2 Antibody (3B8) [H00083667-M03]

Immunohistochemistry-Paraffin: SESN2 Antibody (3B8) [H00083667-M03]

Immunohistochemistry-Paraffin: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of monoclonal antibody to SESN2 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml
Western Blot: SESN2 Antibody (3B8) [H00083667-M03]

Western Blot: SESN2 Antibody (3B8) [H00083667-M03]

Western Blot: SESN2 Antibody (3B8) [H00083667-M03] - SESN2 expression in K-562 ( Cat # L009V1 ).
Immunoprecipitation: SESN2 Antibody (3B8) [H00083667-M03]

Immunoprecipitation: SESN2 Antibody (3B8) [H00083667-M03]

Immunoprecipitation: SESN2 Antibody (3B8) [H00083667-M03] - SESN2 transfected lysate.
Immunoprecipitation: SESN2 Antibody (3B8) [H00083667-M03]

Immunoprecipitation: SESN2 Antibody (3B8) [H00083667-M03]

Immunoprecipitation: SESN2 Antibody (3B8) [H00083667-M03] - Analysis of SESN2 transfected lysate using anti-SESN2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SESN2 rabbit polyclonal antibody.
Sandwich ELISA: SESN2 Antibody (3B8) [H00083667-M03] - Detection limit for recombinant GST tagged SESN2 is approximately 0.3ng/ml as a capture antibody.

Applications for SESN2 Antibody (3B8) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SESN2

This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions.

Alternate Names

DKFZp761M0212, DKFZp761M02121, Hi95, HI95sestrin-2, SES2, SEST2hypoxia induced gene 95, sestrin 2

Entrez Gene IDs

83667 (Human)

Gene Symbol

SESN2

OMIM

607767 (Human)

UniProt

AAH13304 (Human)

Additional SESN2 Products

Product Documents for SESN2 Antibody (3B8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SESN2 Antibody (3B8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SESN2 Antibody (3B8) - Azide and BSA Free

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Protocols

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FAQs

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