SG2NA Antibody (S68) - BSA Free

Novus Biologicals | Catalog # NB110-74572

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S68

Format

BSA Free
View all SG2NA Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human SG2NA (within residues 200-300). [Swiss-Prot# Q13033]

Localization

Cytoplasm and membrane.

Specificity

This antibody is specific for SG2NA. It does not cross-react with Striatin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SG2NA Antibody (S68) - BSA Free

Western Blot: SG2NA Antibody (S68) [NB110-74572]

Western Blot: SG2NA Antibody (S68) [NB110-74572]

Western Blot: SG2NA Antibody (S68) [NB110-74572] - Detection of SG2NA in NIH3T3 cell lysates using NB110-74572.
Immunocytochemistry/ Immunofluorescence: SG2NA Antibody (S68) [NB110-74572]

Immunocytochemistry/ Immunofluorescence: SG2NA Antibody (S68) [NB110-74572]

Immunocytochemistry/Immunofluorescence: SG2NA Antibody (S68) [NB110-74572] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SG2NA (S68) [NB110-74572] at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/ Immunofluorescence: SG2NA Antibody (S68) [NB110-74572]

Immunocytochemistry/ Immunofluorescence: SG2NA Antibody (S68) [NB110-74572]

Immunocytochemistry/Immunofluorescence: SG2NA Antibody (S68) [NB110-74572] - Immunofluorescent staining of NIH3T3 cells using NB110-74572.

Applications for SG2NA Antibody (S68) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunoprecipitation

1:10-1:500

Western Blot

1:250-1:2000
Application Notes
This SG2NA antibody is useful in Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB a band can be seen at ~90 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 3 reviews rated 4.3 using NB110-74572 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SG2NA

SG2NA was identified as a human cancer autoantigen and has now been implicated in vesicular trafficking and signal transduction. It is highly related to striatin and zinedin. It is a scaffolding protein that binds PP2A A and C subunits as well as the human homolog of the yeast protein, Mob1, which has been implicated in control of mitotic progression in yeast. It also binds calmodulin and appears to modulate PP2A activity.

Alternate Names

Cell cycle autoantigen SG2NA, cell cycle S/G2 nuclear autoantigen, GS2NA, S/G2 antigen, SG2NAnuclear autoantigen, striatin, calmodulin binding protein 3, striatin-3

Entrez Gene IDs

29966 (Human); 94186 (Mouse); 114520 (Rat)

Gene Symbol

STRN3

UniProt

Q13033 (Human)

Additional SG2NA Products

Product Documents for SG2NA Antibody (S68) - BSA Free

Certificate of Analysis

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Product Specific Notices for SG2NA Antibody (S68) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SG2NA Antibody (S68) - BSA Free

Customer Reviews for SG2NA Antibody (S68) - BSA Free (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • SG2NA Antibody (S68)
    Name: Katarzyna Nalecz
    Application: Proximity Ligation Assay (PLA)
    Sample Tested: Paraformeldehyde Fixed Cells
    Species: Rat
    Verified Customer | Posted 01/25/2017
    SG2NA phosphorylation in rat astrocytes detected with anti-phosphoserine antibody and NB110-74572
    SG2NA Antibody (S68) - BSA Free NB110-74572
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T lysate
    Species: Human
    Verified Customer | Posted 05/11/2016
    Striatin-3 knockdown with siRNA (Lanes1-3:Scramble,3 day, and 4 day)
    SG2NA Antibody (S68) - BSA Free NB110-74572
  • Name: Cynthia Lam
    Application: Western Blot
    Sample Tested: 293T lysate
    Species: Human
    Verified Customer | Posted 05/11/2016
    293T lysate Western Blot and Striatin-3 RNAi
    SG2NA Antibody (S68) - BSA Free NB110-74572

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Protocols

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FAQs for SG2NA Antibody (S68) - BSA Free

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  • Q: What is the concentration of this antibody?

    A: Unfortunately, we have not determined the concentration for this antibody. Please note that none of unpurified antibodies derived directly from ascites is subjected to the concentration determination. This is because there are so many IgG-unrelated proteins that are present in ascites, and the readings could reach to the double-digit in mg/ml. Therefore the usefulness of this kind of antibodies are indicated as the dilution factors for the different applications, after lab-testings

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