SGC UBD253

Key Product Details

Product Description

SGC UBD253 is a potent and selective histone deacetylase 6 (HDAC6-UBD) antagonist (K_D = 80 nM, 84 nM and 95 nM determined by ITC, SPR and FP, respectively, for the UBD domain; K_D = 0.26 μM and 0.44 μM determined by SPR and FP, respectively, for full length HDAC6). Displays 50-fold selectivity for HDAC6 over other UBD proteins, with the exception of USP16 (15-fold). In live cells, it significantly decreases the interaction between HDAC6 and ISG15 (EC₅₀ = 1.9 μM in a NanoBRET assay). Treatment with SGC UBD253 does not inhibit the catalyitic activity of HDAC6.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the SGC UBD253 probe summary on the SGC website.

External Portal Information

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of SGC UBD253 is reviewed on the chemical probes website.

Product Specifications for SGC UBD253

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for SGC UBD253

The following data is based on the product molecular weight 429.86.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.01 mM	232.63 mL	1,163.17 mL	2,326.34 mL
0.05 mM	46.53 mL	232.63 mL	465.27 mL
0.1 mM	23.26 mL	116.32 mL	232.63 mL
0.5 mM	4.65 mL	23.26 mL	46.53 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Harding Discovery and characterization of a chemical probe targeting the zinc-finger ubiquitin-binding domain of HDAC6. J.Med.Chem. 2023 PMID: 37499118

Product Documents for SGC UBD253