Siglec-10 Antibody (265817) [PerCP]
Novus Biologicals | Catalog # FAB2130C
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
PerCP (Excitation = 490 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 265817
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-10
Met17-Thr546
Accession # Q96LC7
Met17-Thr546
Accession # Q96LC7
Specificity
Detects human Siglec-10 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Siglec-11 is observed. In Western blots (reducing conditions), approximately 25% with rhSiglec-11 is observed. In direct ELISAs and Western blots, no cross-reactivity with rhSiglec-2, -3, -5, -7, -9, or recombinant mouse Siglec-F is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for Siglec-10 Antibody (265817) [PerCP]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB2130. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Siglec-10
Long Name
Sialic Acid Binding Ig-like Lectin 10
Alternate Names
Siglec10, SLG2
Gene Symbol
SIGLEC10
Additional Siglec-10 Products
Product Documents for Siglec-10 Antibody (265817) [PerCP]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Siglec-10 Antibody (265817) [PerCP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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