Simple Plex Discovery Human FCRL5/FcRH5 Carridge

R&D Systems | Catalog # SPCKB-PS-016663

Simple Plex Discovery Human FCRL5 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by PS (Plasma/Serum).
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

5.66 pg/mL

Assay Range

13.1-20000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human FCRL5/FcRH5 Carridge Summary

Simple Plex Discovery assay is for the detection of human Fc Receptor-Like 5 (FCRL5/FcRH5) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all FCRL5 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge with 3 Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human FCRL5/FcRH5.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one FCRL5/FcRH5 assay.

Inter-Assay Precision (Precision between assays) Replicates of each FCRL5/FcRH5 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human FCRL5/FcRH5.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 28 28
Mean (pg/mL) 151 7583 141 6916
Standard Deviation 9.98 275 11.0 595
CV% 6.6 3.6 7.8 8.6

Recovery for Simple Plex Discovery Human FCRL5/FcRH5 Carridge

Recovery for the human FCRL5/FcRH5 assays was evaluated at three different spiked concentrations of FCRL5/FcRH5 protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 107 103-111

Linearity

Samples containing and/or spiked with high concentrations of FCRL5/FcRH5 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human FCRL5/FcRH5 Assay Linearity

Scientific Data Images for Simple Plex Discovery Human FCRL5/FcRH5 Carridge

Simple Plex Discovery Human FCRL5/FcRH5 Assay Standard Curve

Simple Plex Discovery Human FCRL5/FcRH5 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human FCRL5/FcRH5 Assay. Assay range is 13.1-20,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FCRL5

Fc receptor homologs, also known as IRTA or IFGP (IgSF, FcR, gp42) proteins, are transmembrane proteins with sequence homology to classical Fc receptors. FcRH have from three to nine Ig-like domains in their extracellular region and variable combinations of cytoplasmic immunotyrosine activation or inhibitory motifs (ITAM or ITIM). FcRH function as activating or inhibitory receptors on discrete B cell populations and are involved in lymphocyte development and transformation. No ligands for these proteins have been identified.

Long Name

Fc Receptor-like 5

Alternate Names

BXMAS1, CD307e, FcRH5, IFGP5, IRTA2, PRO820

Entrez Gene IDs

83416 (Human); 329693 (Mouse)

Gene Symbol

FCRL5

Additional FCRL5 Products

Product Documents for Simple Plex Discovery Human FCRL5/FcRH5 Carridge

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human FCRL5/FcRH5 Carridge

For research use only

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