Simple Plex Discovery Human Serpin B2/PAI-2 Cartridge

R&D Systems | Catalog # SPCKB-PS-016665

Simple Plex Discovery Human Serpin B2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by PS (Plasma/Serum).
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

73.6 pg/mL

Assay Range

164-40000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human Serpin B2/PAI-2 Cartridge Summary

Simple Plex Discovery assay is for the detection of human Serpin B2 (also known as Plasminogen Activator Inhibitor 2, PAI-2) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) samples using Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Serpin B2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge with 3 Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Serpin B2/PAI-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Serpin B2/PAI-2 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Serpin B2/PAI-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Serpin B2/PAI-2.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 21 22
Mean (pg/mL) 244 12168 253 12553
Standard Deviation 13.3 392 25.0 610
CV% 5.5 3.2 9.9 4.9

Recovery for Simple Plex Discovery Human Serpin B2/PAI-2 Cartridge

Recovery for the human Serpin B2/PAI-2 assays was evaluated at three different spiked concentrations of Serpin B2/PAI-2 protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 105 99-110
EDTA Plasma (n=2) 112 111-113
Heparin Plasma (n=2) 115 111-120
Serum (n=2) 117 115-120

Linearity

Samples containing and/or spiked with high concentrations of Serpin B2/PAI-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human Serpin B2/PAI-2 Assay Linearity

Scientific Data Images for Simple Plex Discovery Human Serpin B2/PAI-2 Cartridge

Simple Plex Discovery Human Serpin B2/PAI-2 Assay Standard Curve

Simple Plex Discovery Human Serpin B2/PAI-2 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Serpin B2/PAI-2 Assay. Assay range is 164-40,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin B2

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Alternate Names

PAI2, PLANH2, Urokinase Inhibitor

Entrez Gene IDs

5055 (Human); 18788 (Mouse); 60325 (Rat)

Gene Symbol

SERPINB2

Additional Serpin B2 Products

Product Documents for Simple Plex Discovery Human Serpin B2/PAI-2 Cartridge

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human Serpin B2/PAI-2 Cartridge

For research use only

Citations for Simple Plex Discovery Human Serpin B2/PAI-2 Cartridge

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