Simple Plex Discovery Human vWF-A2 Cartridge

R&D Systems | Catalog # SPCKB-PS-016135

Simple Plex Discovery Human vWF-A2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by PS (Plasma/Serum).
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

1.29 pg/mL

Assay Range

13.1-20000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human vWF-A2 Cartridge Summary

Simple Plex Discovery assay is for the detection of human von Willebrand Factor A2 domain (vWF-A2) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) samples using Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all vWF-A2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge with 3 Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human vWF-A2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one vWF-A2 assay.

Inter-Assay Precision (Precision between assays) Replicates of each vWF-A2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human vWF-A2.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 43 44
Mean (pg/mL) 164 7310 154 7067
Standard Deviation 6.16 442 18.1 669
CV% 3.8 6.0 11.8 9.5

Recovery for Simple Plex Discovery Human vWF-A2 Cartridge

Recovery for the human vWF-A2 assays was evaluated at three different spiked concentrations of vWF-A2 protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 111 111-112
EDTA Plasma (n=2) 93 91-94
Heparin Plasma (n=2) 102 98-105
Serum (n=2) 90 89-91

Linearity

Samples containing and/or spiked with high concentrations of vWF-A2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human vWF-A2 Assay Linearity

Scientific Data Images for Simple Plex Discovery Human vWF-A2 Cartridge

Simple Plex Discovery Human vWF-A2 Assay Standard Curve

Simple Plex Discovery Human vWF-A2 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human vWF-A2 Assay. Assay range is 13.1-20,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: vWF-A2

Human vWF is a serum glycoprotein that mediates platelet adhesion to injured vascular endothelium. vWF function is regulated by ADAMTS13 mediated proteolysis within the A2 domain. Mutations of this protease cause thrombotic thrombocytopenic purpura.

Long Name

von Willebrand Factor A2 Domain

Alternate Names

F8VWF, vWFA2

Entrez Gene IDs

7450 (Human); 22371 (Mouse); 116669 (Rat)

Gene Symbol

VWF

Additional vWF-A2 Products

Product Documents for Simple Plex Discovery Human vWF-A2 Cartridge

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human vWF-A2 Cartridge

For research use only

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Associated Pathways

Blood Coagulation Signaling Pathways
Blood Coagulation Signaling Pathway Thumbnail