SMAC/Diablo Antibody (78-1-118) - BSA Free

Novus Biologicals | Catalog # NB500-213

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 78-1-118

Format

BSA Free
View all SMAC/Diablo Primary Antibodies »

Product Specifications

Immunogen

Recombinant mature human Smac (lacks amino acids 1-55 present in Smac precursor). [UniProt# Q9NR28]

Localization

Mitochondrial but released into the cytosol when cells undergo apoptosis.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMAC/Diablo Antibody (78-1-118) - BSA Free

Western Blot: SMAC/Diablo Antibody (78-1-118) [NB500-213]

Western Blot: SMAC/Diablo Antibody (78-1-118) [NB500-213]

Western Blot: Smac Antibody (78-1-118) [NB500-213] - HeLa whole cell extract lysates.

Applications for SMAC/Diablo Antibody (78-1-118) - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500
Application Notes
This Smac (78-1-118) antibody is useful for Immunoprecipitation and Western blot, where a band is seen at ~25 kDa. For better results, IP prior to Western blot is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 4 reviews rated 4.8 using NB500-213 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMAC/Diablo

Smac/DIABLO, a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enters the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms (2,3).

Long Name

Second Mitochondria-derived Activator of Caspase

Alternate Names

Diablo

Entrez Gene IDs

56616 (Human); 66593 (Mouse); 288753 (Rat)

Gene Symbol

DIABLO

UniProt

Q9NR28

Additional SMAC/Diablo Products

Product Documents for SMAC/Diablo Antibody (78-1-118) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SMAC/Diablo Antibody (78-1-118) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for SMAC/Diablo Antibody (78-1-118) - BSA Free

Customer Reviews for SMAC/Diablo Antibody (78-1-118) - BSA Free (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • SMAC/Diablo Antibody (78-1-118)
    Name: PRAVEEN Dubey
    Application: Western Blot
    Sample Tested: H9c2 whole cell lysate
    Species: Rat
    Verified Customer | Posted 02/07/2022
    SMAC
    SMAC/Diablo Antibody (78-1-118) - BSA Free NB500-213
  • SMAC/Diablo Antibody (78-1-118)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: AC!6 and AC16
    Species: Human
    Verified Customer | Posted 04/20/2021
    SMAC
    SMAC/Diablo Antibody (78-1-118) - BSA Free NB500-213
  • SMAC/Diablo Antibody (78-1-118)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: AC16
    Species: Mouse
    Verified Customer | Posted 05/15/2019
    SMAC/DIABLO
    SMAC/Diablo Antibody (78-1-118) - BSA Free NB500-213
  • SMAC/Diablo Antibody (78-1-118)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: AC16
    Species: Human
    Verified Customer | Posted 05/06/2019
    DIABLO
    SMAC/Diablo Antibody (78-1-118) - BSA Free NB500-213

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Showing  1 - 4 of 4 reviews Showing All

Protocols

View specific protocols for SMAC/Diablo Antibody (78-1-118) - BSA Free (NB500-213):

SMAC/Diablo Antibody (78-1-118):
Procedure Guide for NB 500-213 Monoclonal anti-Survivin (clone 58.22)

Immunoprecipitation Procedure
1) Lyse cells plated in a 60mm dish:
a) 300 ul CHAPS buffer [50mM Tris-HCl, pH 7.5/50mM NaCl/1mM EDTA/1% NP-40/0.1% CHAPS/1mM NaVO4/1mM PMSF]
b) rock for 20 minutes at 4C
2) Harvest lysate and spin down the insoluble material at 14K rpm.
3) Collect soluble fraction.
4) Pre-clear lysate with 40 ul of 50:50 slurry of Protein A beads, rocking for 1 hour at 4C.
5) Spin down beads at 2K rpm, at 4C.
6) Collect pre-cleared lysate.
7) Incubate lysate with 5-7ug of anti-Survivin (NB 500-213) overnight, rocking at 4C.
8) Add 50 ul of Protein A 50:50 slurry for 2 hours, rocking at 4C.
9) Wash beads with 200 ul of CHAPS buffer, three times.
10) Denature immune complex by adding 2x Sample Buffer, containing 2-ME.
11) Boil for 10 minutes and load onto an SDS-gel.

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail