SMAD1/9 Antibody (4E12H10) - BSA Free

Novus Biologicals | Catalog # NBP2-61878

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4E12H10

Format

BSA Free
View all SMAD1/9 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human SMAD1/9 (AA: 1-110) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMAD1/9 Antibody (4E12H10) - BSA Free

Western Blot: SMAD1/9 Antibody (4E12H10)BSA Free [NBP2-61878]

Western Blot: SMAD1/9 Antibody (4E12H10)BSA Free [NBP2-61878]

Western Blot: SMAD1/9 Antibody (4E12H10) [NBP2-61878] - Analysis using SMAD1 mAb against HEK293 (1) and SMAD1 (AA: 1-110)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: SMAD1/9 Antibody (4E12H10)BSA Free [NBP2-61878]

Western Blot: SMAD1/9 Antibody (4E12H10)BSA Free [NBP2-61878]

Western Blot: SMAD1/9 Antibody (4E12H10) [NBP2-61878] - Analysis using SMAD1 mAb against human SMAD1 (AA: 1-110) recombinant protein. (Expected MW is 38.5 kDa)
Western Blot: SMAD1/9 Antibody (4E12H10)BSA Free [NBP2-61878]

Western Blot: SMAD1/9 Antibody (4E12H10)BSA Free [NBP2-61878]

Western Blot: SMAD1/9 Antibody (4E12H10) [NBP2-61878] - Analysis using SMAD1 mouse mAb against NIH/3T3 (1), COS7 (2), HUVEC (3), and C2C12 (4) cell lysate.
ELISA: SMAD1/9 Antibody (4E12H10) - BSA Free [NBP2-61878]

ELISA: SMAD1/9 Antibody (4E12H10) - BSA Free [NBP2-61878]

ELISA: SMAD1/9 Antibody (4E12H10) [NBP2-61878] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for SMAD1/9 Antibody (4E12H10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMAD1/9

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.;

Alternate Names

BSP1, BSP-1, hSMAD1, JV41, JV4-1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1, MADH1JV41, MADR1, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1

Gene Symbol

SMAD1

Additional SMAD1/9 Products

Product Documents for SMAD1/9 Antibody (4E12H10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SMAD1/9 Antibody (4E12H10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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