SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25516

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (92%), Chimpanzee (100%), Rat (92%), Rhesus Macaque (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1460 expressed in HEK293

Format

Azide and BSA Free
View all SOD2/Mn-SOD Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys68 of human SOD2/Mn-SOD. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (84%), Xenopus (84%).

Modification

ac Lys 68

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free

SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free

Western Blot: SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free [NBP3-25516] -

Western Blot: SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free [NBP3-25516] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (NBP3-25516) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free

Western Blot: SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free [NBP3-25516] -

Western Blot: SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free [NBP3-25516] - Whole cell extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (NBP3-25516) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free

Dot Blot: SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) [NBP3-25516] -

Dotblot analysis of anti-SOD2/Mn-SOD antibody [HL1460] with peptide samples. Peptide samples were spotted onto positively charged nylon membrane and blotted with SOD2/Mn-SOD antibody [HL1460] (NBP3-25516) varied dilution.

Applications for SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOD2/Mn-SOD

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Long Name

Superoxide Dismutase-2

Alternate Names

IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial

Gene Symbol

SOD2

Additional SOD2/Mn-SOD Products

Product Documents for SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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