![Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050]](https://resources.rndsystems.com/images/products/SOX10-Antibody-CL4455-Immunohistochemistry-NBP2-59050-img0002.jpg "Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL4455
Format
BSA Free
Loading...
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P56693, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for SOX10 Antibody (CL4455) - BSA Free
Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050]
Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing trigeminal ganglion and ear placode.![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]](https://resources.rndsystems.com/images/products/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0004.jpg "Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]")
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cerebral cortex shows strong nuclear immunoreactivity in a subset of glial cells.![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]](https://resources.rndsystems.com/images/products/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0005.jpg "Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]")
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human breast shows strong nuclear positivity in glandular cells.![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]](https://resources.rndsystems.com/images/products/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0009.jpg "Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]")
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cervix shows nuclear immunoreactivity in a subset of basal cells in squamous epithelium.
SOX10 Antibody (CL4455) [NBP2-59050] -
Staining of mouse embryo E11 shows strong positivity in the developing trigeminal ganglion.![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]](https://resources.rndsystems.com/images/products/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0007.jpg "Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]")
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human pancreas shows absence of positivity as expected (negative control).![SOX10 Antibody (CL4455) - BSA Free Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050]](https://resources.rndsystems.com/images/products/nbp2-59050_-immunocytochemistry-immunofluorescence-639174077030746277.jpg "Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050]")
Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050]
Staining of SK-MEL-30 cells, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).Applications for SOX10 Antibody (CL4455) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:2500 - 1:5000
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Reviewed Applications
Read 1 review rated 3 using NBP2-59050 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SOX10
Long Name
SRY-box transcription factor 10
Alternate Names
DOM, PCWH, SOX-10, WS2E, WS4, WS4C
Gene Symbol
SOX10
Additional SOX10 Products
Product Documents for SOX10 Antibody (CL4455) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SOX10 Antibody (CL4455) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for SOX10 Antibody (CL4455) - BSA Free (1)
3 out of 5
1 Customer Rating
Have you used SOX10 Antibody (CL4455) - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Customer Images
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Immunohistochemistry-FrozenSample Tested: Adult spinal cordSpecies: FelineVerified Customer | Posted 03/14/2019Image was captured using fluorescent microscopy demonstrating SOX10 (red) and DAPI (blue).For NBP2-59050 antibody, I used it at 1:200 in 1X PBS + 0.05% tween 20+ 2% BSA. As I tested it at 1:50, 1:100, 1:200, 1:500 and 1:1000, that concentration gave the best signal/noise ratio for IHC-Fr. The slides were subsequently incubated with alexa fluor conjugated secondary antibody for 1hr at RT and imaged with fluorescent microscopy.
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
