SOX9 Antibody (MSVA-709R)

Novus Biologicals | Catalog # NBP3-57254

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-709R
View all SOX9 Primary Antibodies »

Product Specifications

Immunogen

Recombinant humanSOX9 protein fragment (around aa 393- 508) (exact sequence is proprietary) Swissprot P48436

Localization

Nucleus

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Colon: A strong SOX9 immunostaining should be seen in epithelial cells of the crypt base..

Negative Control
Negitve control:Colon: SOX9 immunostaining should be absent in all non-epithelial cell types (except few cells in lymphoid germinal centres – if present).

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SOX9 Antibody (MSVA-709R)

SOX9 Antibody (MSVA-709R)

Immunohistochemistry-Paraffin:SOX9 Antibody (MSVA-709R)NBP3-57254

Testis Strong SOX9 staining of Sertoli cells 1
SOX9 Antibody (MSVA-709R)

Immunohistochemistry-Paraffin:SOX9 Antibody (MSVA-709R)NBP3-57254

Esophagus – Squamous cell carcinoma with strong SOX9 immunostaining of most tumor cells 1
SOX9 Antibody (MSVA-709R)

Immunohistochemistry-Paraffin:SOX9 Antibody (MSVA-709R)NBP3-57254

Duodenum mucosa

Applications for SOX9 Antibody (MSVA-709R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SOX9

SOX9 is a member of the family of SOX (Sry-type highmobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Gene Symbol

SOX9

Additional SOX9 Products

Product Documents for SOX9 Antibody (MSVA-709R)

Certificate of Analysis

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Product Specific Notices for SOX9 Antibody (MSVA-709R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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