StAR Antibody (MSVA-740R)

Novus Biologicals | Catalog # NBP3-57268

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-740R
View all StAR Primary Antibodies »

Product Specifications

Immunogen

Recombinant human STAR fragment Swissprot P49675

Localization

Intracellular

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Adrenal gland: A moderate to strong cytoplasmic StAR staining should be seen in the vast majority of adrenocortical cells..

Negative Control
Negitve control:Colon: All cells must not show any StAR staining.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for StAR Antibody (MSVA-740R)

StAR Antibody (MSVA-740R)

Immunohistochemistry-Paraffin:StAR Antibody (MSVA-740R)NBP3-57268

Adrenocortical carcinoma showing strong STAR immunostaining of tumor cells
StAR Antibody (MSVA-740R)

Immunohistochemistry-Paraffin:StAR Antibody (MSVA-740R)NBP3-57268

Strong cytoplasmic StAR immunostaining of Leydig cells
StAR Antibody (MSVA-740R)

Immunohistochemistry-Paraffin:StAR Antibody (MSVA-740R)NBP3-57268

Strong StAR staining of normal adrenocortical cells

Applications for StAR Antibody (MSVA-740R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: StAR

StAR is encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.

Alternate Names

cholesterol trafficker, mitochondrial steroid acute regulatory protein, StAR, StARD1, STARD1StAR-related lipid transfer (START) domain containing 1, START domain containing 1, START domain-containing protein 1, steroid acute regulatory protein, steroidogenic acute regulator, steroidogenic acute regulatory protein, steroidogenic acute regulatory protein, mitochondrial

Gene Symbol

STAR

Additional StAR Products

Product Documents for StAR Antibody (MSVA-740R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for StAR Antibody (MSVA-740R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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