Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN-α). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.
STAT5a/b Antibody (3J2A2)
Novus Biologicals | Catalog # NBP3-16533
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Scientific Data Images for STAT5a/b Antibody (3J2A2)
Western Blot: STAT5a/b Antibody (3J2A2) [NBP3-16533]
Western Blot: STAT5a/b Antibody (3J2A2) [NBP3-16533] - Western blot analysis of extracts of various cell lines, using STAT5a/b Rabbit mAb (NBP3-16533) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Applications for STAT5a/b Antibody (3J2A2)
Western Blot
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Background: STAT5a/b
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Additional STAT5a/b Products
Product Documents for STAT5a/b Antibody (3J2A2)
Certificate of Analysis
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Product Specific Notices for STAT5a/b Antibody (3J2A2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars