STAT5b Antibody (6P5T0)

Name: STAT5b Antibody (6P5T0)
Brand: Novus Biologicals
SKU: NBP3-15778
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15778

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6P5T0 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 600-787 of human STAT5B (P51692). KQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCESATAKAVDGYVKPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STAT5b Antibody (6P5T0)

Western Blot: STAT5b Antibody (6P5T0) [NBP3-15778]

Western Blot: STAT5b Antibody (6P5T0) [NBP3-15778] - Western blot analysis of extracts of various cell lines, using STAT5b antibody (NBP3-15778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western Blot: STAT5b Antibody (6P5T0) [NBP3-15778]

Western Blot: STAT5b Antibody (6P5T0) [NBP3-15778] - Western blot analysis of extracts from normal (control) and STAT5b knockout (KO) HeLa cells, using STAT5b antibody (NBP3-15778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation: STAT5b Antibody (6P5T0) [NBP3-15778]

Immunoprecipitation: STAT5b Antibody (6P5T0) [NBP3-15778] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug STAT5b antibody (NBP3-15778). Western blot was performed from the immunoprecipitate using STAT5b antibody (NBP3-15778) at a dilition of 1:1000.

Applications for STAT5b Antibody (6P5T0)

Application

Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT5b

STAT5B is encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

Long Name

Signal Transducer and Activator of Transcription 5b

Alternate Names

signal transducer and activator of transcription 5B, STAT5, transcription factor STAT5B

Gene Symbol

STAT5B

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Product Documents for STAT5b Antibody (6P5T0)

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Certificate of Analysis

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Product Specific Notices for STAT5b Antibody (6P5T0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.