STK33 Antibody (6G1) - Azide and BSA Free

Novus Biologicals | Catalog # H00065975-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6G1

Format

Azide and BSA Free
View all STK33 Primary Antibodies »

Product Specifications

Immunogen

STK33 (AAH31231, 1 a.a. ~ 514 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADSGLDKKSTKCPDCSSASQKDVLCVCSSKTRVPPVLVVEMSQTSSIGSAESLISLERKKEKNINRDITSRKDLPSRTSNVERKASQQQWGRGNFTEGKVPHIRIENGAAIEEIYTFGRILGKGSFGIVIEATDKETETKWAIKKVNKEKAGSSAVKLLEREVNILKSVKHEHIIHLEQVFETPKKMYLVMELCEDGELKEILDRKGHFSENETRWIIQSLASAIAYLHNNDIVHRDLKLENIMVKSSLIDDNNEINLNIKVTDFGLAVKKQSRSEAMLQATCGTPIYMAPEVISAHDYSQQCDIWSIGVVMYMLLRGEPPFLASSEGKLFELIRKGELHFENAVWNSISDCAKSVLKQLMKVDPAHRITAKELLDNQWLTGNKLSSVRPTNVLEMMKEWKNNPESVEENTTEEKNKPSTEEKLKSYQPWGNVPETNYTSDEEEEKQSTTYEKQFPATSKDNFDMCSSSFTSSKLLPAEIKGEMEKTPVTPSQGTATKYPAKSGALSRTKKKL

Reactivity Notes

Human. Other species not tested.

Specificity

STK33 - serine/threonine kinase 33

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for STK33 Antibody (6G1) - Azide and BSA Free

Western Blot: STK33 Antibody (6G1) [H00065975-M02]

Western Blot: STK33 Antibody (6G1) [H00065975-M02]

Western Blot: STK33 Antibody (6G1) [H00065975-M02] - STK33 monoclonal antibody (M02), clone 6G1 Analysis of STK33 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: STK33 Antibody (6G1) [H00065975-M02]

Immunocytochemistry/ Immunofluorescence: STK33 Antibody (6G1) [H00065975-M02]

Immunocytochemistry/Immunofluorescence: STK33 Antibody (6G1) [H00065975-M02] - Analysis of monoclonal antibody to STK33 on HeLa cell. Antibody concentration 25 ug/ml.
Western Blot: STK33 Antibody (6G1) [H00065975-M02]

Western Blot: STK33 Antibody (6G1) [H00065975-M02]

Western Blot: STK33 Antibody (6G1) [H00065975-M02] - Analysis of STK33 over-expressed 293 cell line, cotransfected with STK33 Validated Chimera RNAi ( Cat # H00065975-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STK33 monoclonal antibody (M02) clone 6G1 (Cat # H00065975-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: STK33 Antibody (6G1) [H00065975-M02]

Western Blot: STK33 Antibody (6G1) [H00065975-M02]

Western Blot: STK33 Antibody (6G1) [H00065975-M02] - Analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody (M02), clone 6G1.Lane 1: STK33 transfected lysate(57.9 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: STK33 Antibody (6G1) [H00065975-M02]

Immunoprecipitation: STK33 Antibody (6G1) [H00065975-M02]

Immunoprecipitation: STK33 Antibody (6G1) [H00065975-M02] - Analysis of STK33 transfected lysate using anti-STK33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK33 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: STK33 Antibody (6G1) [H00065975-M02] - Detection limit for recombinant GST tagged STK33 is 1 ng/ml as a capture antibody.

Applications for STK33 Antibody (6G1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, RNAi validation, and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STK33

Serine/threonine Kinase 33 (STK33) is expressed at high levels in testis and the fetal lung and heart, and at lower levels in a number of other tissues. However, STK33 is different form the other serine/threonine kinases in that it is not expressed in neural tissues. STK33 is thought to be involved in the phosphorylation of vimentin. Additionally, STK33 has been identified as a synthetic lethal in KRAS mutant lung cancers.

Long Name

Serine/threonine-protein kinase 33

Alternate Names

EC 2.7.11, EC 2.7.11.1, serine/threonine kinase 33, serine/threonine-protein kinase 33

Entrez Gene IDs

65975 (Human)

Gene Symbol

STK33

OMIM

607670 (Human)

UniProt

AAH31231 (Human)

Additional STK33 Products

Product Documents for STK33 Antibody (6G1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for STK33 Antibody (6G1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for STK33 Antibody (6G1) - Azide and BSA Free

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Protocols

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FAQs

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